{"product_id":"zebrafish-pou5f1-antibody-pou5f3-bha17136470","title":"Zebrafish Pou5f1 Antibody \/ Pou5f3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Pou5f1 Antibody \/ Pou5f3 is a anti-POU5F1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POU5F1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePou5f1 (POU domain, class 5, transcription factor 1), also known as Oct4, is a critical transcription factor that plays a central role in maintaining pluripotency and self-renewal in embryonic stem cells. It regulates a network of genes that control cell fate determination, stem cell maintenance, and differentiation during early development. As a member of the POU family of transcription factors, Pou5f1 binds to specific DNA sequences to modulate gene expression. It is essential for the regulation of pluripotent stem cells and for ensuring proper embryonic development in many organisms, including zebrafish.\u003cp\u003eIn zebrafish, Pou5f1 is an ortholog of the human POU5F1 gene (also known as Oct4). The zebrafish and human versions of Pou5f1 share high sequence homology, particularly in their POU domain, which is the functional region responsible for DNA binding and transcriptional regulation. This conservation makes zebrafish a suitable model for studying stem cell pluripotency, embryogenesis, and developmental processes. Pou5f1 is expressed during early stages of embryonic development, where it plays a key role in maintaining stem cell populations and preventing premature differentiation.\u003c\/p\u003e\n\u003cp\u003eZebrafish Pou5f1 has isoforms, which may have distinct functions or expression patterns depending on the stage of development or tissue type. These isoforms enable fine-tuned regulation of transcription, particularly in early development, ensuring that the pluripotent state of embryonic cells is maintained while allowing differentiation when necessary. The diversity in isoforms is important for the regulation of gene networks and cellular behaviors during embryonic patterning and organogenesis.\u003c\/p\u003e\n\u003cp\u003eIn addition to its role as an essential transcription factor in early development, Pou5f1 is related to the Pou5f3 protein in zebrafish. While both proteins belong to the same POU family of transcription factors, they have distinct but overlapping roles in development. Pou5f3, like Pou5f1, is involved in early developmental stages and stem cell regulation. However, Pou5f3 has been shown to play a specific role in the development of the nervous system and neuronal differentiation, whereas Pou5f1 is more broadly involved in the regulation of pluripotency and self-renewal across multiple cell types. Both proteins share sequence similarity in their POU domain but diverge in their tissue-specific functions and regulatory roles during development.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POU5F1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POU5F1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POU5F1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POU5F1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POU5F1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331291501,"sku":"RZ1275","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_15a71cb0-f882-4a21-9b3d-5c8b3f7f95a6.jpg?v=1772019469","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-pou5f1-antibody-pou5f3-bha17136470","provider":"BioHippo","version":"1.0","type":"link"}