{"product_id":"zebrafish-ppm1g-antibody-protein-phosphatase-1g-bha17136472","title":"Zebrafish Ppm1g Antibody \/ Protein phosphatase 1G","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Ppm1g Antibody \/ Protein phosphatase 1G is a anti-PPM1G Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPM1G\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePpm1g (Protein phosphatase 1G) is a member of the protein phosphatase 1 family, which plays a crucial role in cellular signaling, cell cycle regulation, and stress responses. This protein is involved in the dephosphorylation of target proteins, which is an essential process for regulating cellular processes such as growth, proliferation, and differentiation. Ppm1g specifically acts to remove phosphate groups from serine\/threonine residues in its substrate proteins, influencing various signal transduction pathways and metabolic processes.\u003cp\u003eIn zebrafish, Ppm1g is an ortholog of the human PPM1G gene. The zebrafish and human proteins show significant sequence conservation, particularly in their phosphatase domain, which is crucial for their catalytic activity. This conservation suggests that Ppm1g performs similar roles in signal transduction and cellular regulation across species, making zebrafish a valuable model for studying protein dephosphorylation, cell cycle control, and signal transduction mechanisms.\u003c\/p\u003e\n\u003cp\u003ePpm1g in zebrafish may also have isoforms, which can vary in their tissue-specific expression and functional roles. These isoforms allow for dynamic regulation of protein phosphorylation during different developmental stages, cellular responses, and physiological conditions. Isoform diversity can contribute to the fine-tuning of cell signaling pathways, especially during embryonic development and stress responses.\u003c\/p\u003e\n\u003cp\u003eZebrafish Ppm1g is expressed in several tissues, including the brain, heart, and liver, where it plays a role in cellular homeostasis and the regulation of signal transduction pathways involved in metabolism, growth, and development. Its expression is crucial for maintaining protein phosphorylation levels that ensure proper cell cycle progression and stress responses. Disruptions in Ppm1g function in zebrafish can lead to defects in cellular regulation, development, and stress tolerance, providing a useful model for studying diseases related to protein phosphorylation and cell cycle dysfunction.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPM1G - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPM1G - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPM1G - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPM1G - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPM1G+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330177389,"sku":"RZ1277","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_86f5b478-9c68-457b-a82e-638e79818fd8.jpg?v=1772019469","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-ppm1g-antibody-protein-phosphatase-1g-bha17136472","provider":"BioHippo","version":"1.0","type":"link"}