{"product_id":"zebrafish-ppp1r12a-antibody-mypt1-bha17136439","title":"Zebrafish Ppp1r12a Antibody \/ Mypt1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Ppp1r12a Antibody \/ Mypt1 is a anti-PPP1R12A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPP1R12A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePpp1r12a, or protein phosphatase one regulatory subunit twelve a, is a regulatory protein that controls the activity of protein phosphatase one, a major serine threonine phosphatase involved in numerous cellular processes. In zebrafish, Ppp1r12a plays a critical role in regulating cytoskeletal organization, cell contractility, and morphogenesis during development.\u003cp\u003eZebrafish Ppp1r12a is the ortholog of the human PPP1R12A gene, which encodes the protein also known as myosin phosphatase target subunit one. This protein directs protein phosphatase one to myosin light chains and other cytoskeletal targets, enabling dynamic control of actomyosin contraction and cell movement. It is involved in signaling pathways that govern smooth muscle relaxation, cell shape changes, and cell migration.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish development, Ppp1r12a is expressed in tissues undergoing active morphogenetic movements and structural rearrangement, such as the developing somites, neural tube, and heart. It contributes to proper tissue organization by regulating the phosphorylation state of cytoskeletal components and by modulating mechanical tension within cells.\u003c\/p\u003e\n\u003cp\u003eDisruption of Ppp1r12a function in zebrafish has been shown to affect cell adhesion, motility, and organ formation, highlighting its importance in developmental processes. Due to its conservation across vertebrates and its central role in regulating phosphatase activity, zebrafish Ppp1r12a is a useful model for studying signal transduction, cytoskeletal regulation, and developmental disorders involving cell morphology and contractility.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPP1R12A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPP1R12A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPP1R12A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPP1R12A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPP1R12A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329718637,"sku":"RZ1244","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_621d88e1-f2c2-408e-b467-c8f6a28c2c5f.jpg?v=1772019465","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-ppp1r12a-antibody-mypt1-bha17136439","provider":"BioHippo","version":"1.0","type":"link"}