{"product_id":"zebrafish-prox1a-antibody-prospero-homeobox-1a-bha17136475","title":"Zebrafish Prox1a Antibody \/ Prospero homeobox 1a","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Prox1a Antibody \/ Prospero homeobox 1a is a anti-PROX1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PROX1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eProx1a (Prospero-related homeobox 1a) is a transcription factor that plays a critical role in the regulation of cell fate determination, organogenesis, and tissue differentiation during embryonic development. It is part of the homeobox gene family and functions primarily as a nuclear transcriptional regulator involved in the development of the central nervous system, eye, liver, pancreas, and lymphatic vasculature.\u003cp\u003eIn zebrafish, Prox1a is one of 2 paralogs, the other being Prox1b, both arising from a genome duplication event. Prox1a is highly conserved and serves as an ortholog of the human PROX1 gene. The human and zebrafish proteins share conserved domains, including the homeodomain and prospero domain, which are essential for DNA binding and transcriptional regulation. This conservation supports the use of zebrafish as a model for studying human developmental biology and disease mechanisms involving PROX1.\u003c\/p\u003e\n\u003cp\u003eZebrafish Prox1a has known isoforms resulting from alternative splicing. These isoforms may differ in regulatory or functional capacities and show distinct expression patterns during various stages of development. Isoform diversity allows for specialized roles in different tissues and developmental contexts.\u003c\/p\u003e\n\u003cp\u003eProx1a is expressed in several key regions during zebrafish embryogenesis, including the central nervous system, retina, liver primordium, and developing lymphatic vessels. It is essential for the development of the liver and the specification of lymphatic endothelial cells, functioning as a master regulator of lymphangiogenesis. Loss of Prox1a function in zebrafish results in defects in organ development and disrupted lymphatic vessel formation.\u003c\/p\u003e\n\u003cp\u003eGiven its fundamental roles in organ development and lymphatic specification, zebrafish Prox1a is widely used to study transcriptional regulation, stem cell differentiation, and vascular biology. It is also a valuable model for understanding congenital diseases and cancer progression involving the lymphatic system and liver.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PROX1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PROX1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PROX1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PROX1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PROX1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331389805,"sku":"RZ1280","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b7c07010-854e-454e-80ac-68ef5a927924.jpg?v=1772019470","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-prox1a-antibody-prospero-homeobox-1a-bha17136475","provider":"BioHippo","version":"1.0","type":"link"}