{"product_id":"zebrafish-prpf18-antibody-pre-mrna-splicing-factor-18-bha17136476","title":"Zebrafish Prpf18 Antibody \/ Pre-mRNA-splicing factor 18","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Prpf18 Antibody \/ Pre-mRNA-splicing factor 18 is a anti-PRPF18 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRPF18\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePrpf18 (Pre-mRNA processing factor 18) is a key component of the spliceosome, the large ribonucleoprotein complex responsible for the removal of introns from precursor messenger RNA during gene expression. Prpf18 plays an essential role in the second catalytic step of splicing, contributing to the proper assembly and function of the spliceosomal complex and ensuring the accuracy of mRNA processing.\u003cp\u003eIn zebrafish, Prpf18 is an ortholog of the human PRPF18 gene. The zebrafish and human proteins are highly conserved, particularly within domains essential for spliceosomal interactions and catalytic activity. This conservation suggests functional equivalency between species, making zebrafish a relevant model for studying pre-mRNA splicing and its regulation in development and disease.\u003c\/p\u003e\n\u003cp\u003eZebrafish Prpf18 may have isoforms arising from alternative splicing events, although isoform-specific functions are still under investigation. The presence of multiple isoforms may allow differential regulation of splicing in a tissue-specific or developmental stage-specific manner.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish embryogenesis, Prpf18 is broadly expressed, with notable enrichment in rapidly dividing and transcriptionally active tissues such as the neural tube and developing somites. Its expression is crucial for early development, as disruption of splicing machinery can lead to widespread defects in gene regulation, cell cycle control, and differentiation.\u003c\/p\u003e\n\u003cp\u003eGiven its central role in RNA splicing, zebrafish Prpf18 is important for studies related to gene expression regulation, developmental biology, and spliceosome-associated disorders. It also serves as a useful model for understanding human genetic diseases caused by splicing defects, including certain neurodegenerative and retinal disorders.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRPF18 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRPF18 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRPF18 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRPF18 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRPF18+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330472301,"sku":"RZ1281","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a8a7250e-6248-4f36-bc39-1bb59fd32f54.jpg?v=1772019470","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-prpf18-antibody-pre-mrna-splicing-factor-18-bha17136476","provider":"BioHippo","version":"1.0","type":"link"}