{"product_id":"zebrafish-prpf31-antibody-prp31-pre-mrna-processing-factor-31-bha17136477","title":"Zebrafish Prpf31 Antibody \/ Prp31 \/ Pre-mRNA processing factor 31","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Prpf31 Antibody \/ Prp31 \/ Pre-mRNA processing factor 31 is a anti-PRPF31 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRPF31\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePrpf31 (Pre-mRNA processing factor 31) is a core component of the spliceosome, the complex responsible for removing introns from pre-messenger RNA transcripts. It is essential for the proper assembly and stability of the U4\/U6-U5 tri-snRNP complex, a critical step in the formation of the mature spliceosome. By enabling efficient and accurate splicing of pre-mRNA, Prpf31 supports normal gene expression and cellular function.\u003cp\u003eIn zebrafish, Prpf31 is the ortholog of the human PRPF31 gene. The zebrafish and human proteins are highly conserved, especially in functional domains that mediate interactions with other spliceosomal proteins. This conservation suggests that Prpf31 in zebrafish plays equivalent roles in pre-mRNA splicing and is suitable for modeling human splicing-related conditions.\u003c\/p\u003e\n\u003cp\u003eZebrafish Prpf31 may have multiple isoforms due to alternative splicing. These isoforms can contribute to functional diversity and may exhibit distinct spatial or temporal expression patterns during development. Isoform-specific roles are under ongoing investigation and may offer insights into tissue-specific splicing regulation.\u003c\/p\u003e\n\u003cp\u003eExpression of Prpf31 in zebrafish is detected in tissues with high levels of transcriptional activity, such as the developing eye, brain, and somites. During early embryogenesis, its presence is crucial for the proper expression of genes required for organ development and cell differentiation.\u003c\/p\u003e\n\u003cp\u003eMutations in the human PRPF31 gene are associated with autosomal dominant retinitis pigmentosa, a degenerative eye disease. Zebrafish models have been used to study the pathogenesis of this condition and to explore therapeutic strategies, due to the gene’s conserved function and retinal expression.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRPF31 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRPF31 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRPF31 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRPF31 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRPF31+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330537837,"sku":"RZ1282","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_374acc9f-6d5e-4758-b222-57286e78d3ac.jpg?v=1772019469","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-prpf31-antibody-prp31-pre-mrna-processing-factor-31-bha17136477","provider":"BioHippo","version":"1.0","type":"link"}