{"product_id":"zebrafish-psma1-antibody-proteasome-subunit-alpha-type-1-bha17136479","title":"Zebrafish Psma1 Antibody \/ Proteasome subunit alpha type 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psma1 Antibody \/ Proteasome subunit alpha type 1 is a anti-PSMA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMA1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsma1 (Proteasome subunit alpha type 1) is a core component of the 20S proteasome, a highly conserved protein complex responsible for the degradation of ubiquitinated proteins within the cell. Psma1 belongs to the alpha ring of the proteasome core particle, which plays a structural role and helps regulate substrate entry into the proteolytic chamber of the proteasome. The proteasome is essential for maintaining protein homeostasis, regulating the cell cycle, modulating immune responses, and facilitating the removal of damaged or misfolded proteins.\u003cp\u003eIn zebrafish, Psma1 is the ortholog of the human PSMA1 gene. The zebrafish and human proteins share strong sequence conservation, especially in regions critical for proteasome assembly and function. This conservation highlights the functional equivalence of Psma1 across species and supports the utility of zebrafish as a model organism for investigating proteasome-related pathways and diseases.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psma1 may have isoforms resulting from alternative splicing. These isoforms could exhibit differences in regulatory sequences or tissue distribution, potentially contributing to specialized roles in specific developmental stages or cell types. However, the primary isoform is typically the dominant functional variant in most tissues.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish development, Psma1 is ubiquitously expressed, reflecting the universal need for regulated protein degradation in cellular maintenance. Expression is particularly enriched in proliferative and metabolically active tissues such as the brain, liver, and musculature. The function of Psma1 is critical for embryo viability, as disruption of proteasome function can lead to accumulation of toxic protein aggregates and defects in signaling pathways.\u003c\/p\u003e\n\u003cp\u003eIn human biology, proteasome subunit mutations, including those in PSMA1, have been associated with neurodegenerative conditions and immune dysregulation. Because of the high functional conservation, zebrafish Psma1 serves as a powerful tool for modeling these disorders, studying proteasome biology, and evaluating potential pharmacological modulators of proteasome activity.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMA1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMA1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMA1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMA1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMA1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330898285,"sku":"RZ1284","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c426b270-5524-4b1b-a2d4-b4c6dfa1a730.jpg?v=1772019471","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psma1-antibody-proteasome-subunit-alpha-type-1-bha17136479","provider":"BioHippo","version":"1.0","type":"link"}