{"product_id":"zebrafish-psma3-antibody-proteasome-subunit-alpha-type-3-bha17136480","title":"Zebrafish Psma3 Antibody \/ Proteasome subunit alpha type 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psma3 Antibody \/ Proteasome subunit alpha type 3 is a anti-PSMA3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMA3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsma3 (Proteasome subunit alpha type 3) is a structural component of the 20S core particle of the proteasome, a large multi-protein complex responsible for the degradation of intracellular proteins. Psma3 belongs to the alpha subunit family, which forms the outer rings of the barrel-shaped proteasome structure. These alpha subunits serve primarily as a gate for substrate entry and help regulate access to the proteolytically active beta subunits located inside the proteasome core.\u003cp\u003eIn zebrafish, Psma3 is an ortholog of the human PSMA3 gene. The zebrafish and human proteins are highly conserved in both sequence and structure, especially in domains critical for proteasome assembly and function. This conservation supports the use of zebrafish as a reliable model for studying proteasome dynamics, protein turnover, and diseases related to impaired proteostasis.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psma3 may exist in multiple isoforms generated through alternative splicing. These isoforms can differ in their regulatory elements, cellular localization, or tissue-specific expression patterns, although the predominant isoform is typically involved in the canonical function of proteasome assembly and protein degradation.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish embryogenesis, Psma3 is broadly expressed across many tissue types, reflecting the fundamental role of the proteasome in maintaining cellular protein quality. Expression is particularly high in metabolically active tissues such as the developing brain, eye, liver, and somites. The protein is essential for embryonic growth, tissue morphogenesis, and cellular stress responses.\u003c\/p\u003e\n\u003cp\u003eIn human systems, dysfunction of PSMA3 or related proteasome components has been linked to disorders such as cancer, neurodegeneration, and autoinflammatory diseases. Given the high degree of conservation, zebrafish Psma3 is a valuable model for investigating the role of the proteasome in normal physiology and in the pathogenesis of diseases involving impaired protein degradation or ubiquitin-proteasome system dysfunction.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMA3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMA3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMA3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMA3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMA3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331422573,"sku":"RZ1285","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8b76a175-7991-41cd-b678-24cc2e834f8c.jpg?v=1772019471","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psma3-antibody-proteasome-subunit-alpha-type-3-bha17136480","provider":"BioHippo","version":"1.0","type":"link"}