{"product_id":"zebrafish-psma5-antibody-proteasome-subunit-alpha-type-5-bha17136481","title":"Zebrafish Psma5 Antibody \/ Proteasome subunit alpha type 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psma5 Antibody \/ Proteasome subunit alpha type 5 is a anti-PSMA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMA5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsma5 (Proteasome subunit alpha type 5) is a structural component of the 20S core particle of the proteasome, a key intracellular complex responsible for the ATP-independent degradation of ubiquitinated proteins. Psma5 belongs to the alpha subunit family, which forms the two outer rings of the proteasome core. These alpha subunits regulate substrate entry into the proteolytic chamber formed by the beta subunits and are essential for proteasome assembly, stability, and function.\u003cp\u003eIn zebrafish, Psma5 is the ortholog of the human PSMA5 gene. The protein sequence and functional domains are highly conserved between zebrafish and humans, indicating a shared role in proteasome-mediated protein degradation. This evolutionary conservation supports the use of zebrafish as a model organism for studying proteostasis, developmental regulation, and disease mechanisms related to proteasome dysfunction.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psma5 may have multiple isoforms generated through alternative splicing. These isoforms could allow tissue-specific or developmental stage-specific regulation of proteasome activity, although the canonical isoform is primarily involved in the assembly and function of the standard 20S proteasome.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish embryogenesis, Psma5 is broadly expressed in rapidly proliferating and metabolically active tissues, including the developing brain, eye, musculature, and digestive organs. Its expression reflects the universal need for controlled protein degradation in processes such as cell cycle progression, signal transduction, and removal of damaged or misfolded proteins.\u003c\/p\u003e\n\u003cp\u003eIn human biology, defects in proteasome components including PSMA5 have been associated with neurodegenerative diseases, cancers, and immune system disorders. Zebrafish Psma5, due to its conserved function, serves as a valuable model for functional studies of the proteasome and for the development of therapeutics targeting the ubiquitin-proteasome system.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMA5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMA5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMA5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMA5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMA5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330439533,"sku":"RZ1286","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c2a3604a-9b58-460e-a738-a1bb39598911.jpg?v=1772019472","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psma5-antibody-proteasome-subunit-alpha-type-5-bha17136481","provider":"BioHippo","version":"1.0","type":"link"}