{"product_id":"zebrafish-psmb1-antibody-proteasome-subunit-beta-type-1-bha17136482","title":"Zebrafish Psmb1 Antibody \/ Proteasome subunit beta type 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmb1 Antibody \/ Proteasome subunit beta type 1 is a anti-PSMB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMB1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmb1 (Proteasome subunit beta type 1) is a critical structural component of the 20S core particle within the proteasome complex. The proteasome is essential for the regulated degradation of intracellular proteins, particularly those tagged with ubiquitin. Psmb1 is one of the seven beta subunits that form the inner rings of the 20S proteasome and is involved in maintaining the structural integrity and assembly of the complex, although it does not possess catalytic activity itself.\u003cp\u003eIn zebrafish, Psmb1 is an ortholog of the human PSMB1 gene. The zebrafish and human proteins are highly conserved in sequence and function, indicating evolutionary preservation of this essential proteasome subunit. This makes zebrafish a suitable model organism for investigating proteasome biology and related human diseases.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psmb1 may have isoforms resulting from alternative splicing, although the predominant isoform is primarily responsible for incorporation into the core proteasome structure. These potential isoforms may contribute to tissue-specific regulation of proteasome function or be expressed under certain developmental or stress conditions.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish development, Psmb1 is ubiquitously expressed, reflecting the widespread requirement for protein turnover in cellular homeostasis, growth, and differentiation. Expression is especially prominent in tissues with high metabolic activity, such as the brain, eye, muscle, and liver. Disruption of Psmb1 expression or proteasome assembly can impair embryonic development and lead to cellular stress due to the accumulation of damaged or misfolded proteins.\u003c\/p\u003e\n\u003cp\u003eIn humans, alterations in proteasome function including mutations in PSMB1 have been implicated in disorders such as neurodegeneration, cancer, and autoimmune syndromes. Zebrafish Psmb1 is therefore a valuable research target for understanding the roles of the proteasome in development, disease, and therapeutic intervention strategies that modulate proteasomal activity.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMB1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMB1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMB1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMB1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMB1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330734445,"sku":"RZ1287","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6fd65627-db45-4004-81ae-d612b575a27d.jpg?v=1772019471","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmb1-antibody-proteasome-subunit-beta-type-1-bha17136482","provider":"BioHippo","version":"1.0","type":"link"}