{"product_id":"zebrafish-psmc1-antibody-psmc1a-psmc1b-26s-proteasome-regulatory-subunit-4-bha17136483","title":"Zebrafish Psmc1 Antibody \/ Psmc1a \/ Psmc1b \/ 26S proteasome regulatory subunit 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmc1 Antibody \/ Psmc1a \/ Psmc1b \/ 26S proteasome regulatory subunit 4 is a anti-PSMC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMC1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmc1 (Proteasome 26S subunit ATPase 1) is a crucial component of the 19S regulatory particle of the 26S proteasome complex, which is responsible for recognizing, unfolding, and translocating polyubiquitinated substrates into the 20S core for degradation. As an ATPase, Psmc1 belongs to the AAA family of ATPases and contributes mechanical energy required for substrate processing, making it essential for proteasome-mediated proteolysis.\u003cp\u003eIn zebrafish, Psmc1 is the ortholog of the human PSMC1 gene. The zebrafish and human proteins show strong sequence and functional conservation, especially in the ATPase domain and other motifs critical for proteasome assembly and activity. This high degree of conservation supports the use of zebrafish as a model to study proteasomal function and its relevance to human disease.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psmc1 may have multiple isoforms generated through alternative splicing. These isoforms can exhibit variation in domain composition or expression patterns and may play specific roles during different stages of development or under stress conditions. However, the canonical isoform is primarily responsible for forming the regulatory ATPase ring of the proteasome.\u003c\/p\u003e\n\u003cp\u003eDuring zebrafish embryogenesis, Psmc1 is broadly expressed and particularly enriched in rapidly dividing and metabolically active tissues such as the central nervous system, developing musculature, and gastrointestinal organs. Its role in protein quality control is critical for proper cell cycle progression, apoptosis regulation, and differentiation.\u003c\/p\u003e\n\u003cp\u003eIn humans, mutations or dysregulation of PSMC1 have been associated with proteasome-related disorders and neurodevelopmental syndromes. Given its conserved function, zebrafish Psmc1 serves as an excellent model for exploring the molecular mechanisms of proteasome activity and its impact on developmental and pathological processes.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMC1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMC1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMC1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMC1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMC1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330603373,"sku":"RZ1288","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e3319247-7f9e-488a-bdf4-254ee478f42c.jpg?v=1772019475","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmc1-antibody-psmc1a-psmc1b-26s-proteasome-regulatory-subunit-4-bha17136483","provider":"BioHippo","version":"1.0","type":"link"}