{"product_id":"zebrafish-psmc5-antibody-26s-proteasome-regulatory-subunit-8-bha17136485","title":"Zebrafish Psmc5 Antibody \/ 26S proteasome regulatory subunit 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmc5 Antibody \/ 26S proteasome regulatory subunit 8 is a anti-PSMC5 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMC5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmc5 (Proteasome 26S subunit ATPase 5) is a crucial component of the 26S proteasome, which is involved in the ATP-dependent degradation of ubiquitinated proteins. As a member of the AAA ATPase family, Psmc5 plays a significant role in recognizing and unfolding substrates before they are translocated into the 20S catalytic core of the proteasome for proteolysis. This function is essential for maintaining cellular homeostasis by regulating protein turnover, cell cycle progression, and response to stress.\u003cp\u003eIn zebrafish, Psmc5 is the ortholog of the human PSMC5 gene, and they share high sequence identity as well as functional conservation. The zebrafish and human proteins possess conserved domains critical for ATP hydrolysis and substrate processing, making zebrafish an excellent model for studying proteasome function.\u003c\/p\u003e\n\u003cp\u003eLike its human counterpart, Psmc5 in zebrafish may have isoforms resulting from alternative splicing. These isoforms could exhibit differences in regulatory subunit composition or functional roles within the proteasomal complex, especially in specialized tissues or during developmental stages.\u003c\/p\u003e\n\u003cp\u003eExpression of Psmc5 in zebrafish is widespread, with notable presence in actively dividing cells, such as those in the brain, heart, and digestive tissues. This distribution underscores its essential role in development and cellular maintenance.\u003c\/p\u003e\n\u003cp\u003eIn humans, mutations or dysregulation of PSMC5 have been associated with various diseases, including neurodegenerative disorders and cancers, often due to impaired proteasomal degradation. Zebrafish Psmc5 serves as an ideal model for studying proteasome-related diseases and for screening potential therapeutic agents that target the proteasome machinery.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMC5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMC5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMC5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMC5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMC5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331258733,"sku":"RZ1290","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f5372093-8a40-475e-a27e-d4080ffa5cab.jpg?v=1772019474","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmc5-antibody-26s-proteasome-regulatory-subunit-8-bha17136485","provider":"BioHippo","version":"1.0","type":"link"}