{"product_id":"zebrafish-psmd13-antibody-26s-proteasome-non-atpase-regulatory-subunit-13-bha17136486","title":"Zebrafish Psmd13 Antibody \/ 26S proteasome non-ATPase regulatory subunit 13","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmd13 Antibody \/ 26S proteasome non-ATPase regulatory subunit 13 is a anti-PSMD13 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD13\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmd13 (Proteasome 26S subunit, non-ATPase 13) is a key component of the 26S proteasome complex, which is responsible for the ATP-dependent degradation of ubiquitinated proteins. It is part of the 19S regulatory particle, which controls the recognition, unfolding, and translocation of substrates into the 20S catalytic core for degradation. Psmd13 plays an essential role in the regulation of the proteasomal function and is involved in maintaining cellular homeostasis by controlling the turnover of a wide variety of regulatory and signaling proteins.\u003cp\u003eIn zebrafish, Psmd13 is the ortholog of the human PSMD13 gene, sharing high sequence identity and functional conservation between the two species. The zebrafish protein has similar domains responsible for interacting with other proteasomal components and regulatory factors, ensuring proper proteasome assembly and activity.\u003c\/p\u003e\n\u003cp\u003eLike its human counterpart, Psmd13 in zebrafish may have isoforms, likely arising from alternative splicing events. These isoforms may play specialized roles in different tissues or developmental stages, potentially influencing the efficiency of protein degradation in various cellular contexts. Such isoforms could also have tissue-specific expression patterns, contributing to the regulation of cell-cycle progression, stress responses, and signal transduction.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Psmd13 is highly expressed in tissues with active cell division, such as the brain, liver, and kidney, where proteasomal activity is essential for maintaining protein turnover and cellular integrity. This expression pattern aligns with the protein’s conserved role in protein quality control, cellular differentiation, and apoptosis regulation.\u003c\/p\u003e\n\u003cp\u003eThe study of Psmd13 in zebrafish offers valuable insights into proteasome-related diseases in humans, such as neurodegenerative diseases and cancers, where disruptions in proteasomal degradation pathways can lead to the accumulation of damaged proteins. Zebrafish serves as an excellent model for studying the molecular mechanisms of proteasomal dysfunction and for screening potential therapeutic agents aimed at modulating proteasome activity.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD13 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD13 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD13 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD13 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD13+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330767213,"sku":"RZ1291","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9769dec8-04e2-42e6-9398-de13467db467.jpg?v=1772019472","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmd13-antibody-26s-proteasome-non-atpase-regulatory-subunit-13-bha17136486","provider":"BioHippo","version":"1.0","type":"link"}