{"product_id":"zebrafish-psmd14-antibody-26s-proteasome-non-atpase-regulatory-subunit-14-bha17136466","title":"Zebrafish Psmd14 Antibody \/ 26S proteasome non-ATPase regulatory subunit 14","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmd14 Antibody \/ 26S proteasome non-ATPase regulatory subunit 14 is a anti-PSMD14 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD14\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmd14 (Proteasome 26S subunit, non-ATPase 14) is a protein that is a part of the 26S proteasome complex, which is responsible for the degradation of ubiquitinated proteins. The 26S proteasome plays a crucial role in regulating cellular protein turnover, maintaining protein homeostasis, and regulating processes such as cell cycle progression, signal transduction, DNA repair, and gene expression. Psmd14, specifically, is involved in the regulation of proteasomal activity and is a key component of the regulatory particle of the proteasome.\u003cp\u003eIn zebrafish, Psmd14 is an ortholog of the human PSMD14 gene. Both zebrafish and human Psmd14 share substantial sequence conservation, particularly in their role within the 26S proteasome complex, ensuring functional equivalence in protein degradation. This high degree of similarity makes zebrafish a valuable model organism for studying the molecular mechanisms of protein degradation and its impact on various cellular processes, including development, growth, and disease progression.\u003c\/p\u003e\n\u003cp\u003ePsmd14 is expressed in a variety of tissues in zebrafish, including the brain, heart, and muscles, reflecting its importance in general cellular maintenance and metabolic processes. It has a critical role in regulating ubiquitin-mediated proteolysis, which is essential for cell cycle regulation, especially during embryonic development, cell differentiation, and tissue regeneration.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psmd14 has isoforms, which may vary in their expression patterns and functional properties. These isoforms are likely to have tissue-specific roles, particularly in highly dynamic tissues such as neural and muscle tissue. The presence of isoforms suggests a degree of functional specialization and adaptability to specific cellular contexts, allowing Psmd14 to be involved in diverse aspects of protein homeostasis and stress responses.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD14 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD14 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD14 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD14 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD14+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331357037,"sku":"RZ1271","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e287a411-f3ee-4960-84cd-bdcb2925a628.jpg?v=1772019467","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmd14-antibody-26s-proteasome-non-atpase-regulatory-subunit-14-bha17136466","provider":"BioHippo","version":"1.0","type":"link"}