{"product_id":"zebrafish-psmd2-antibody-26s-proteasome-non-atpase-regulatory-subunit-2-bha17136487","title":"Zebrafish Psmd2 Antibody \/ 26S proteasome non-ATPase regulatory subunit 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmd2 Antibody \/ 26S proteasome non-ATPase regulatory subunit 2 is a anti-PSMD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmd2 (Proteasome 26S subunit, non-ATPase 2) is a crucial component of the 26S proteasome, a multi-subunit complex responsible for the ATP-dependent degradation of ubiquitinated proteins. It is part of the 19S regulatory particle of the proteasome, which facilitates the recognition, unfolding, and translocation of substrates into the 20S catalytic core for proteolysis. Psmd2 plays a key role in regulating protein turnover, influencing the cell cycle, and maintaining cellular homeostasis by controlling the degradation of a wide range of regulatory and signaling proteins.\u003cp\u003eIn zebrafish, Psmd2 is the ortholog of the human PSMD2 gene, showing significant sequence similarity and functional conservation between the two species. The zebrafish Psmd2 protein shares essential domains responsible for interaction with other proteasomal subunits and regulatory factors, enabling the proper assembly and function of the proteasome complex.\u003c\/p\u003e\n\u003cp\u003ePsmd2 in zebrafish may also have isoforms resulting from alternative splicing events. These isoforms may differ in their tissue-specific expression or regulatory roles, potentially affecting proteasomal activity in distinct developmental stages or cellular contexts. The diversity of these isoforms allows for fine-tuned regulation of protein degradation in response to various cellular stresses, such as oxidative stress or DNA damage.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Psmd2 is expressed in a wide range of tissues, particularly in those with high cell turnover and protein synthesis, such as the brain, liver, and kidney. This pattern of expression reflects the essential role of Psmd2 in maintaining protein quality control, regulating the degradation of misfolded or damaged proteins, and modulating signaling pathways critical for cellular functions.\u003c\/p\u003e\n\u003cp\u003eThe zebrafish model, with its conserved proteasomal components, is valuable for studying proteasome-related diseases in humans. Disruptions in PSMD2 function are associated with various human disorders, including neurodegenerative diseases and cancers, where defective protein degradation leads to the accumulation of abnormal proteins. Zebrafish Psmd2 serves as an excellent model for understanding the mechanisms of proteasomal dysfunction and for screening potential therapies targeting the proteasome.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330406765,"sku":"RZ1292","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_04927f6f-80fd-4f7f-b6e6-1c65631cb54b.jpg?v=1772019471","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmd2-antibody-26s-proteasome-non-atpase-regulatory-subunit-2-bha17136487","provider":"BioHippo","version":"1.0","type":"link"}