{"product_id":"zebrafish-psmd4-antibody-psmd4a-psmd4b-26s-proteasome-non-atpase-regulatory-subunit-4-bha17136488","title":"Zebrafish Psmd4 Antibody \/ Psmd4a \/ Psmd4b \/ 26S proteasome non-ATPase regulatory subunit 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmd4 Antibody \/ Psmd4a \/ Psmd4b \/ 26S proteasome non-ATPase regulatory subunit 4 is a anti-PSMD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmd4 (Proteasome 26S subunit, non-ATPase 4) is an integral part of the 19S regulatory particle of the 26S proteasome, which plays a crucial role in the ATP-dependent degradation of ubiquitinated proteins. As a non-ATPase subunit, Psmd4 contributes to the recognition, unfolding, and translocation of substrates into the 20S catalytic core of the proteasome for degradation. This process is vital for maintaining cellular homeostasis, regulating the cell cycle, and controlling protein turnover, particularly in response to cellular stress or damage.\u003cp\u003eIn zebrafish, Psmd4 is the ortholog of the human PSMD4 gene, showing a high degree of sequence identity and functional conservation. Both the zebrafish and human proteins share the same basic structural motifs essential for proteasomal function, including interaction domains that allow for proper assembly and regulation of the proteasomal complex.\u003c\/p\u003e\n\u003cp\u003eZebrafish Psmd4 may have isoforms resulting from alternative splicing events, although specific isoform variants may not be fully characterized. These isoforms could potentially exhibit tissue-specific expression or alternative regulatory roles, further contributing to the fine-tuned regulation of proteasomal activity. The functional diversity provided by these isoforms allows for specialized roles in different tissues or developmental stages, ensuring efficient protein degradation and homeostasis under varying conditions.\u003c\/p\u003e\n\u003cp\u003eThe expression of Psmd4 in zebrafish is widespread, with notable expression in tissues that undergo rapid cell division or have high protein turnover, such as the brain, liver, and kidney. This expression profile highlights the critical role of Psmd4 in maintaining protein integrity, regulating cellular stress responses, and ensuring proper protein homeostasis, particularly in rapidly growing or dividing cells.\u003c\/p\u003e\n\u003cp\u003eGiven the functional conservation between zebrafish and human Psmd4, zebrafish serves as an ideal model for studying the molecular mechanisms underlying proteasomal dysfunction in humans. In particular, PSMD4 mutations in humans are associated with certain diseases, including cancer and neurodegenerative disorders, where impaired proteasomal degradation leads to the accumulation of misfolded or damaged proteins. Zebrafish Psmd4 is a valuable tool for investigating proteasome-related diseases and for screening potential therapeutic agents that can modulate proteasomal activity.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330832749,"sku":"RZ1293","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_37d3a8a3-f4c8-4dcf-9f6a-90b745877344.jpg?v=1772019470","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmd4-antibody-psmd4a-psmd4b-26s-proteasome-non-atpase-regulatory-subunit-4-bha17136488","provider":"BioHippo","version":"1.0","type":"link"}