{"product_id":"zebrafish-psmd6-antibody-26s-proteasome-non-atpase-regulatory-subunit-6-bha17136489","title":"Zebrafish Psmd6 Antibody \/ 26S proteasome non-ATPase regulatory subunit 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmd6 Antibody \/ 26S proteasome non-ATPase regulatory subunit 6 is a anti-PSMD6 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nuclear, cytoplasmic, extracellular.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmd6 (Proteasome 26S subunit, non-ATPase 6) is a vital component of the 19S regulatory particle of the 26S proteasome, which is responsible for the ATP-dependent degradation of ubiquitinated proteins. As part of the regulatory particle, Psmd6 helps facilitate the recognition, unfolding, and translocation of substrates into the 20S catalytic core of the proteasome for subsequent degradation. This protein plays an essential role in controlling protein turnover, maintaining cellular homeostasis, and regulating a wide range of biological processes, including the cell cycle, DNA repair, and stress response.\u003cp\u003eIn zebrafish, Psmd6 is the ortholog of the human PSMD6 gene, sharing high sequence identity and functional conservation. Both zebrafish and human Psmd6 proteins feature conserved domains that are crucial for their role in proteasome function, allowing for the proper assembly and regulation of the 26S proteasome complex. The similarity between zebrafish Psmd6 and human PSMD6 makes zebrafish an excellent model for studying proteasomal mechanisms and related diseases.\u003c\/p\u003e\n\u003cp\u003ePsmd6 in zebrafish may also have isoforms arising from alternative splicing. These isoforms may exhibit variations in their expression patterns or regulatory functions, which could influence proteasomal activity in different tissues or stages of development. The presence of such isoforms allows for specific regulation of proteasomal function under different cellular conditions, such as during cellular stress, development, or differentiation.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Psmd6 is expressed in a variety of tissues, particularly in those with high protein turnover, such as the brain, liver, and kidney. This reflects the protein’s essential role in regulating protein degradation and maintaining cellular integrity, particularly in actively dividing cells or tissues under stress.\u003c\/p\u003e\n\u003cp\u003eDue to its functional conservation with human PSMD6, Psmd6 in zebrafish provides an ideal model for investigating proteasomal dysfunctions related to various human diseases, including neurodegenerative disorders, cancer, and immune-related conditions. Disruptions in PSMD6 function can lead to the accumulation of damaged or misfolded proteins, contributing to disease pathogenesis. The zebrafish model facilitates the study of these diseases and offers opportunities for therapeutic screening targeting proteasomal pathways.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330931053,"sku":"RZ1294","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_19e8d379-18e3-411d-a9b4-3f3606963524.jpg?v=1772019472","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmd6-antibody-26s-proteasome-non-atpase-regulatory-subunit-6-bha17136489","provider":"BioHippo","version":"1.0","type":"link"}