{"product_id":"zebrafish-psmd8-antibody-26s-proteasome-non-atpase-regulatory-subunit-8-bha17136491","title":"Zebrafish Psmd8 Antibody \/ 26S proteasome non-ATPase regulatory subunit 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Psmd8 Antibody \/ 26S proteasome non-ATPase regulatory subunit 8 is a anti-PSMD8 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePsmd8 (Proteasome 26S subunit, non-ATPase 8) is an essential component of the 19S regulatory particle of the 26S proteasome, playing a vital role in the ATP-dependent degradation of ubiquitinated proteins. As part of the regulatory complex, Psmd8 is involved in the recognition, unfolding, and translocation of substrates into the 20S catalytic core, where they are degraded into smaller peptides. This process is critical for maintaining cellular homeostasis, regulating the cell cycle, and ensuring the proper turnover of proteins, which helps prevent the accumulation of damaged or misfolded proteins.\u003cp\u003eIn zebrafish, Psmd8 is the ortholog of the human PSMD8 gene, sharing a high degree of sequence conservation and functional similarity. Both the zebrafish and human versions of Psmd8 contain conserved domains required for proteasomal assembly and interaction with other proteasomal subunits, facilitating the proper function of the 26S proteasome complex. The conservation between these species underscores the value of zebrafish as a model organism for studying proteasomal mechanisms and their contribution to human health.\u003c\/p\u003e\n\u003cp\u003ePsmd8 in zebrafish may also have isoforms due to alternative splicing events. These isoforms, while not fully characterized, could exhibit tissue-specific expression or distinct functional roles in different stages of development. Variations in isoforms can allow for the fine-tuning of proteasomal activity in response to different cellular environments, including stress or differentiation signals. The presence of multiple isoforms enhances the adaptability of the proteasome in maintaining cellular balance across various tissues.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Psmd8 is expressed in several tissues, particularly those involved in high protein turnover and cellular division, such as the brain, liver, and kidney. This broad expression pattern highlights its essential role in controlling the degradation of regulatory proteins and supporting cellular processes like growth, stress responses, and apoptosis.\u003c\/p\u003e\n\u003cp\u003eThe functional conservation of Psmd8 between zebrafish and humans makes it an excellent model for understanding the molecular mechanisms underlying proteasomal dysfunctions in humans. Alterations in PSMD8 function have been implicated in various diseases, including neurodegenerative disorders and cancers, where impaired proteasomal degradation leads to the accumulation of toxic proteins. Zebrafish Psmd8 offers a valuable tool for studying these diseases and exploring potential therapeutic strategies aimed at modulating proteasomal activity to treat proteostasis-related disorders.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330636141,"sku":"RZ1296","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c3cfaa79-a3ab-479a-bcb9-66f056ccba9f.jpg?v=1772019473","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-psmd8-antibody-26s-proteasome-non-atpase-regulatory-subunit-8-bha17136491","provider":"BioHippo","version":"1.0","type":"link"}