{"product_id":"zebrafish-rab1a-antibody-rab1aa-rab1ab-bha17136493","title":"Zebrafish Rab1a Antibody \/ Rab1aa \/ Rab1ab","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Rab1a Antibody \/ Rab1aa \/ Rab1ab is a anti-RAB1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAB1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRab1a is a member of the Rab GTPase family, which regulates intracellular vesicle trafficking, membrane dynamics, and cellular processes such as secretion, endocytosis, and organelle maintenance. Rab1a is primarily involved in the regulation of the endoplasmic reticulum (ER) and Golgi apparatus communication. It plays a crucial role in the anterograde transport of proteins from the ER to the Golgi, as well as in the formation of vesicles during this process. It also participates in regulating vesicle docking and fusion, key events in cellular trafficking.\u003cp\u003eIn zebrafish, Rab1a is the ortholog of the human RAB1A gene, with a high degree of sequence conservation and functional similarity. Both the zebrafish and human versions of Rab1a share conserved GTP-binding domains and the mechanisms necessary for vesicle trafficking. The role of Rab1a in zebrafish mirrors its function in humans, supporting processes such as protein secretion, cellular homeostasis, and membrane transport. This functional conservation makes zebrafish an excellent model for studying vesicular trafficking mechanisms and associated diseases.\u003c\/p\u003e\n\u003cp\u003eIn zebrafish, Rab1a exists as Rab1aa and Rab1ab, which are paralogous genes resulting from gene duplication events. Both Rab1aa and Rab1ab encode proteins with similar functions but may exhibit tissue-specific expression patterns or slight differences in their regulatory roles. While Rab1aa and Rab1ab share high sequence homology, they might have distinct roles during development or in various physiological contexts. Isoforms of Rab1a could arise through alternative splicing, allowing for specialized functions in different tissues or developmental stages.\u003c\/p\u003e\n\u003cp\u003eThe Rab1a proteins, including Rab1aa and Rab1ab, play significant roles in cellular processes like vesicle formation, transport, and fusion, especially in tissues with active secretion and protein processing, such as the liver, pancreas, and neurons. These proteins are involved in maintaining the proper function of the ER and Golgi, essential for protein trafficking and cellular signaling. Their roles are particularly important in maintaining the balance of protein homeostasis in cells.\u003c\/p\u003e\n\u003cp\u003eGiven the high conservation between zebrafish Rab1a (including Rab1aa and Rab1ab) and human RAB1A, zebrafish serve as an ideal model for investigating the molecular basis of diseases related to defects in vesicle trafficking, such as neurodegenerative diseases, metabolic disorders, and certain types of cancer. By studying Rab1a and its paralogs, researchers can explore therapeutic strategies aimed at modulating vesicle trafficking pathways, which could help in the treatment of these diseases.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAB1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAB1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAB1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAB1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAB1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331193197,"sku":"RZ1298","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_09eb0567-1e7f-4bfc-b8ea-f94b32101e44.jpg?v=1772019476","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-rab1a-antibody-rab1aa-rab1ab-bha17136493","provider":"BioHippo","version":"1.0","type":"link"}