{"product_id":"zebrafish-rab4a-antibody-rab-4a-bha17136494","title":"Zebrafish Rab4a Antibody \/ Rab-4A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Rab4a Antibody \/ Rab-4A is a anti-RAB4A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAB4A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRab4a is a member of the Rab GTPase family, a large group of small GTP-binding proteins that regulate intracellular vesicle trafficking, membrane dynamics, and several cellular processes such as endocytosis, exocytosis, and intracellular signaling. Specifically, Rab4a is involved in the regulation of early endosome recycling and vesicular trafficking between the endosome and the plasma membrane. It controls the recycling of cargo molecules back to the cell surface, ensuring proper cellular function and maintaining the balance of membrane proteins.\u003cp\u003eIn zebrafish, Rab4a is the ortholog of the human RAB4A gene, sharing a high degree of sequence conservation and performing similar functions in vesicular trafficking and endosome regulation. Both zebrafish Rab4a and human RAB4A are involved in the recycling of membrane receptors and other cargo, which is critical for processes like nutrient uptake, receptor signaling, and maintaining cell surface composition.\u003c\/p\u003e\n\u003cp\u003eWhile Rab4a in zebrafish is well-conserved with its human counterpart, it may exhibit isoforms due to alternative splicing. These isoforms, though not fully characterized, could potentially have tissue-specific expression or variations in functionality based on different cellular contexts or developmental stages. The presence of isoforms of Rab4a could provide a mechanism for fine-tuning the regulation of endosome recycling in different tissues or under various physiological conditions.\u003c\/p\u003e\n\u003cp\u003eRab4a is expressed in tissues that rely heavily on endosomal recycling, such as the liver, kidney, and neurons, where its function in trafficking and receptor recycling plays an important role in cellular homeostasis and communication. In particular, Rab4a is critical for maintaining the flow of membrane components and proteins to ensure normal cellular signaling, nutrient uptake, and overall cell function.\u003c\/p\u003e\n\u003cp\u003eZebrafish Rab4a, due to its high degree of conservation with human RAB4A, is an excellent model for studying endocytic pathways, vesicular trafficking, and diseases associated with defective membrane recycling. Disorders related to abnormal Rab4 function have been linked to defects in endocytosis and cellular signaling, including neurodegenerative diseases, cancer, and metabolic disorders. Studying Rab4a in zebrafish offers valuable insights into these diseases and could aid in the development of therapeutic strategies targeting Rab GTPase pathways.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAB4A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAB4A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAB4A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAB4A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAB4A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330701677,"sku":"RZ1299","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_463aad94-c086-4b9a-a8f0-2442ae56e1d5.jpg?v=1772019472","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-rab4a-antibody-rab-4a-bha17136494","provider":"BioHippo","version":"1.0","type":"link"}