{"product_id":"zebrafish-sdha-antibody-succinate-dehydrogenase-a-bha17136500","title":"Zebrafish Sdha Antibody \/ Succinate dehydrogenase A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Sdha Antibody \/ Succinate dehydrogenase A is a anti-Zebrafish Sdha Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Sdha\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Sdha, also known as succinate dehydrogenase subunit A, is a key component of the mitochondrial respiratory chain complex II. Sdha is an essential flavoprotein that catalyzes the oxidation of succinate to fumarate within the tricarboxylic acid cycle while simultaneously transferring electrons to the electron transport chain. This dual role connects the TCA cycle to oxidative phosphorylation, making Sdha a critical enzyme for cellular energy production.\u003cp\u003eZebrafish Sdha is an ortholog of the human SDHA protein and shares a high degree of sequence and functional conservation. In both species, Sdha functions as the catalytic subunit of succinate dehydrogenase and is required for efficient ATP generation. Mutations or disruptions in Sdha can impair mitochondrial function, leading to metabolic disorders and contributing to pathologies such as cancer and neurodegeneration.\u003c\/p\u003e\n\u003cp\u003eThere is no evidence of multiple isoforms of zebrafish Sdha, and its structure and activity are highly conserved across vertebrates. The zebrafish model provides a valuable system for studying mitochondrial metabolism and diseases related to complex II dysfunction due to the evolutionary similarity to human SDHA.\u003c\/p\u003e\n\u003cp\u003eAntibodies against zebrafish Sdha protein are widely used as mitochondrial markers and for studying metabolic pathways. These antibodies are suitable for applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling the investigation of mitochondrial dynamics and energy metabolism in zebrafish tissues.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Sdha - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Sdha - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Sdha - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Sdha - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Sdha+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047330996589,"sku":"RZ1305","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3edae7aa-4ca7-47dc-b88e-7fd531e5ed02.jpg?v=1772019473","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-sdha-antibody-succinate-dehydrogenase-a-bha17136500","provider":"BioHippo","version":"1.0","type":"link"}