{"product_id":"zebrafish-sf3b3-antibody-splicing-factor-3b-subunit-3-bha17136503","title":"Zebrafish Sf3b3 Antibody \/ Splicing factor 3b subunit 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Sf3b3 Antibody \/ Splicing factor 3b subunit 3 is a anti-SF3B3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P, Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SF3B3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Sf3b3, also known as splicing factor 3B subunit 3, is an essential component of the spliceosome, the molecular machinery responsible for the removal of introns from pre messenger RNA. Sf3b3 is part of the SF3B complex within the U2 small nuclear ribonucleoprotein and plays a crucial role in recognizing and stabilizing the branch point sequence during the splicing process. This activity ensures accurate intron removal and the generation of mature RNA transcripts, which are necessary for proper gene expression.\u003cp\u003eZebrafish Sf3b3 is an ortholog of the human SF3B3 protein, with strong sequence conservation and similar functional roles. Like its human counterpart, zebrafish Sf3b3 is indispensable for the regulation of alternative splicing, which contributes to transcriptome diversity and precise control of protein synthesis. Disruption of Sf3b3 function can have severe effects on embryonic development and cellular homeostasis due to the widespread impact of splicing errors.\u003c\/p\u003e\n\u003cp\u003eCurrently, no distinct isoforms of zebrafish Sf3b3 have been described, but its role as part of the SF3B complex is well established and highly conserved across vertebrate species. The zebrafish model provides a powerful system to study the function of Sf3b3 during development and to investigate splicing defects that may be linked to human diseases.\u003c\/p\u003e\n\u003cp\u003eAntibodies against zebrafish Sf3b3 protein are valuable for studying spliceosome assembly, RNA processing, and transcriptional regulation. These antibodies are commonly applied in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling detailed investigations of splicing factor expression and localization.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SF3B3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SF3B3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SF3B3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SF3B3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SF3B3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331848557,"sku":"RZ1308","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1f626b08-9f33-489c-b0ae-bdddd7a221a8.jpg?v=1772019476","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-sf3b3-antibody-splicing-factor-3b-subunit-3-bha17136503","provider":"BioHippo","version":"1.0","type":"link"}