{"product_id":"zebrafish-shha-antibody-shh-sonic-hedgehog-bha17136504","title":"Zebrafish Shha Antibody \/ Shh \/ Sonic Hedgehog","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Shha Antibody \/ Shh \/ Sonic Hedgehog is a anti-Zebrafish Shha Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm (ER, Golgi), cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Shha\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Shha, also known as sonic hedgehog a, is a signaling molecule that plays a critical role in embryonic development, pattern formation, and cell differentiation. Shha belongs to the hedgehog family of secreted proteins that regulate key developmental pathways, including neural tube patterning, somite organization, and craniofacial morphogenesis. It is particularly important for the proper formation of the central nervous system, muscle segmentation, and the shaping of the midline structures during early development.\u003cp\u003eZebrafish Shha is an ortholog of the human sonic hedgehog (SHH) protein and shares a high level of sequence and functional conservation. Like human SHH, zebrafish Shha undergoes proteolytic processing and lipid modification to produce an active signaling molecule that interacts with the Patched receptor and initiates downstream signaling through the Smoothened and Gli transcription factors. This pathway regulates the expression of numerous target genes that influence cell fate and tissue organization.\u003c\/p\u003e\n\u003cp\u003eThere are no known isoforms of zebrafish Shha, but zebrafish possess another related hedgehog gene called Shhb, which shares overlapping but distinct roles during development. The combination of Shha and Shhb activity helps orchestrate the complex patterning events required for normal embryogenesis.\u003c\/p\u003e\n\u003cp\u003eAntibodies against zebrafish Shha protein are valuable tools for studying hedgehog signaling pathways, developmental biology, and tissue patterning. These antibodies can be applied in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA to monitor Shha expression and localization in embryonic tissues.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Shha - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Shha - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Shha - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Shha - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Shha+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331783021,"sku":"RZ1309","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_136e1b9d-fbb7-442d-a93d-c956d4afac9c.jpg?v=1772019476","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-shha-antibody-shh-sonic-hedgehog-bha17136504","provider":"BioHippo","version":"1.0","type":"link"}