{"product_id":"zebrafish-smarca4a-antibody-smarca4-bha17136505","title":"Zebrafish Smarca4a Antibody \/ Smarca4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Smarca4a Antibody \/ Smarca4 is a anti-SMARCA4A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SMARCA4A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Smarca4a is an ATP dependent chromatin remodeling enzyme that functions as the catalytic core of the Swi Snf type remodeling complexes. By using the energy of ATP hydrolysis, Smarca4a repositions or evicts nucleosomes to regulate access of transcription factors and the transcriptional machinery to DNA. Through this activity Smarca4a controls gene expression programs that are essential for early embryogenesis, tissue differentiation, stem cell maintenance, and responses to developmental and environmental signals.\u003cp\u003eSmarca4a in zebrafish is an ortholog of the human SMARCA4 protein also widely known as Brg1. The zebrafish protein shows strong sequence and functional conservation with the human enzyme, supporting the use of zebrafish as a model to study conserved mechanisms of chromatin remodeling and their roles in development and disease. Zebrafish also carries a related paralog named Smarca4b, which allows comparative analyses of partially overlapping and partially specialized functions within the genome duplication context of teleosts.\u003c\/p\u003e\n\u003cp\u003eSmarca4a is broadly expressed during zebrafish development, with higher levels in rapidly proliferating and differentiating tissues where large scale transcriptional reprogramming is required. Loss of Smarca4a function disrupts body patterning, organogenesis, and cell fate specification, underscoring its central role in controlling chromatin structure and transcription.\u003c\/p\u003e\n\u003cp\u003eAntibodies directed against zebrafish Smarca4a are valuable reagents for studying epigenetic regulation, transcriptional control, and developmental pathways. Typical applications include western blot to assess protein abundance, immunohistochemistry and immunofluorescence to map spatial expression in embryos and tissues, chromatin immunoprecipitation to define genomic binding sites, and immunoprecipitation based assays to characterize Swi Snf complex composition.\u003c\/p\u003e\n\u003cp\u003eBecause zebrafish Smarca4a is an ortholog of human SMARCA4, findings obtained with these antibodies can inform research on human developmental disorders, cancer, and other diseases linked to defects in chromatin remodeling.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SMARCA4A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMARCA4A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SMARCA4A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SMARCA4A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMARCA4A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331094893,"sku":"RZ1310","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_08316eb7-0060-4aab-8425-6739c9ce484e.jpg?v=1772019476","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-smarca4a-antibody-smarca4-bha17136505","provider":"BioHippo","version":"1.0","type":"link"}