{"product_id":"zebrafish-spi1b-antibody-pu-1b-spi1-bha17136509","title":"Zebrafish Spi1b Antibody | Pu.1b | Spi1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Spi1b Antibody | Pu.1b | Spi1 is a anti-SPI1B Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPI1B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Spi1b, also known as PU.1b, is a transcription factor that belongs to the ETS family of DNA binding proteins. Spi1b plays a critical role in the development of hematopoietic lineages, particularly in the differentiation of myeloid and lymphoid cells. It regulates genes involved in immune cell development, function, and response, making it an essential factor for studying blood formation and immune system regulation during zebrafish embryogenesis.\u003cp\u003eZebrafish Spi1b is an ortholog of the human SPI1 protein, also known as PU.1, and shares high sequence and functional conservation. Similar to its human counterpart, Spi1b binds to specific ETS motifs in gene promoters and enhancers to activate or repress target genes. It is particularly important in the early stages of myelopoiesis and is a key regulator of macrophage and neutrophil lineage commitment.\u003c\/p\u003e\n\u003cp\u003eThe use of a Zebrafish Spi1b antibody allows researchers to examine Spi1b expression and localization in developing blood and immune cells. Such antibodies are essential for applications including western blot, immunohistochemistry, and immunofluorescence, enabling detailed characterization of hematopoietic cell populations. A Zebrafish Spi1b antibody can also be used to investigate transcriptional networks controlling immune development and to study the effects of genetic or chemical perturbations on blood cell formation.\u003c\/p\u003e\n\u003cp\u003eThere are no known isoforms of zebrafish Spi1b, and its expression is tightly regulated during embryogenesis and hematopoietic stem cell differentiation. Using a high-quality Zebrafish Spi1b antibody, researchers can gain insights into conserved mechanisms of immune regulation that are relevant to both zebrafish and humans.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPI1B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPI1B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPI1B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPI1B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPI1B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331324269,"sku":"RZ1314","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bee55fe7-f5b1-4032-a688-06bf7f3debc8.jpg?v=1772019475","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-spi1b-antibody-pu-1b-spi1-bha17136509","provider":"BioHippo","version":"1.0","type":"link"}