{"product_id":"zebrafish-srp54-antibody-signal-recognition-particle-54-kda-protein-bha17136511","title":"Zebrafish Srp54 Antibody \/ Signal recognition particle 54 kDa protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Srp54 Antibody \/ Signal recognition particle 54 kDa protein is a anti-SRP54 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SRP54\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Srp54, also known as signal recognition particle 54 kDa protein, is a core component of the signal recognition particle complex that mediates the targeting of newly synthesized proteins to the endoplasmic reticulum membrane. Srp54 binds to the signal sequences of nascent polypeptides and plays an essential role in co translational protein targeting, ensuring that secretory and membrane proteins are properly directed to their correct cellular locations. This function is vital for maintaining protein homeostasis and efficient cellular function.\u003cp\u003eZebrafish Srp54 is an ortholog of the human SRP54 protein and exhibits strong sequence and functional conservation. In both zebrafish and humans, Srp54 contains a GTP binding domain and an M domain that recognizes and interacts with hydrophobic signal sequences. These conserved domains highlight the importance of Srp54 in protein translocation processes that are essential for the development and survival of eukaryotic cells.\u003c\/p\u003e\n\u003cp\u003eThe use of a Zebrafish Srp54 antibody allows researchers to detect and study the distribution and expression of Srp54 during early development and in various tissues. Such antibodies are valuable for western blot, immunohistochemistry, and immunofluorescence applications, providing insights into the role of the SRP pathway in protein targeting. A Zebrafish Srp54 antibody can also be used to analyze the effects of genetic mutations or experimental treatments on protein translocation and endoplasmic reticulum function.\u003c\/p\u003e\n\u003cp\u003eThere are no confirmed isoforms of zebrafish Srp54, and its function appears to be highly conserved across vertebrate species. By using a high-quality Zebrafish Srp54 antibody, researchers can explore the essential roles of the signal recognition particle pathway in protein sorting and its relevance to human biology and disease.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SRP54 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SRP54 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SRP54 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SRP54 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SRP54+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331586413,"sku":"RZ1316","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8bff8e7a-d137-4e17-b7ab-971fe0d81d21.jpg?v=1772019475","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-srp54-antibody-signal-recognition-particle-54-kda-protein-bha17136511","provider":"BioHippo","version":"1.0","type":"link"}