{"product_id":"zebrafish-tbx5a-antibody-tbx5-t-box-protein-5a-bha17136514","title":"Zebrafish Tbx5a Antibody \/ Tbx5 \/ T-box protein 5a","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Tbx5a Antibody \/ Tbx5 \/ T-box protein 5a is a anti-TBX5A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TBX5A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Tbx5a, also known as T-box transcription factor 5a, is a critical regulator of heart and limb development. Tbx5a is a member of the T-box family of transcription factors, which are involved in the regulation of gene expression during embryonic development. It plays a pivotal role in heart morphogenesis, especially in the formation of the cardiac chambers and conduction system, as well as in limb patterning, by controlling the expression of downstream target genes that direct cell fate and differentiation.\u003cp\u003eZebrafish Tbx5a is an ortholog of the human TBX5 protein, sharing significant sequence conservation and functional similarity. In both zebrafish and humans, Tbx5a regulates the formation of the heart's left ventricle and is involved in the proper patterning of the limbs during early development. Mutations in TBX5 in humans are associated with Holt-Oram syndrome, a disorder that leads to congenital heart defects and upper limb abnormalities. The conserved function of zebrafish Tbx5a makes it an ideal model for studying cardiac and limb development and for understanding the underlying genetic causes of congenital malformations.\u003c\/p\u003e\n\u003cp\u003eThe use of a Zebrafish Tbx5a antibody allows researchers to monitor the expression of Tbx5a in various developmental stages, particularly in the heart and limb tissues. These antibodies are invaluable for applications such as western blot, immunohistochemistry, and immunofluorescence, enabling detailed study of Tbx5a expression and its role in patterning and organogenesis. A Zebrafish Tbx5a antibody is also useful for assessing the effects of genetic mutations or environmental factors on heart and limb development.\u003c\/p\u003e\n\u003cp\u003eCurrently, no distinct isoforms of zebrafish Tbx5a have been identified, but its critical role in cardiac and limb development has been well documented. Using a high-quality Zebrafish Tbx5a antibody, researchers can explore the developmental pathways regulated by Tbx5a and gain insights into congenital diseases related to this transcription factor.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TBX5A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TBX5A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TBX5A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TBX5A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TBX5A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331750253,"sku":"RZ1320","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ac13d8a1-1a5a-4f76-ba0a-e271696850b5.jpg?v=1772019474","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-tbx5a-antibody-tbx5-t-box-protein-5a-bha17136514","provider":"BioHippo","version":"1.0","type":"link"}