{"product_id":"zebrafish-tgfb1-antibody-tgfb1a-tgf-beta-bha17136375","title":"Zebrafish Tgfb1 Antibody \/ Tgfb1a \/ Tgf beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Tgfb1 Antibody \/ Tgfb1a \/ Tgf beta is a anti-TGFB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TGFB1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTransforming Growth Factor-beta (TGF-beta) is a multifunctional cytokine that regulates a wide array of biological processes, including cell proliferation, differentiation, apoptosis, and extracellular matrix production. In zebrafish (Danio rerio), multiple tgfb genes have been identified, encoding proteins that are structurally and functionally conserved with their mammalian counterparts.\u003cp\u003eZebrafish TGF-beta proteins belong to the TGF-beta superfamily, which also includes activins, nodal, and bone morphogenetic proteins (BMPs). These ligands signal through serine\/threonine kinase receptors, leading to the phosphorylation of receptor-regulated SMADs (R-Smads), which then form complexes with co-Smad (Smad4) and translocate to the nucleus to regulate gene expression.\u003c\/p\u003e\n\u003cp\u003eTGF-beta signaling in zebrafish is critical during embryogenesis and tissue morphogenesis. It plays essential roles in mesoderm formation, left-right axis specification, cardiovascular development, and immune system modulation. Specific zebrafish isoforms, such as tgfb1a, tgfb1b, and tgfb3, exhibit distinct expression patterns and developmental functions. For example, tgfb1b is involved in hematopoiesis and immune regulation, while tgfb3 contributes to craniofacial development and epithelial integrity.\u003c\/p\u003e\n\u003cp\u003eDue to the genetic tractability and optical transparency of zebrafish embryos, this model system provides unique advantages for in vivo analysis of TGF-β pathway dynamics. Zebrafish TGF-beta proteins serve as valuable tools for studying developmental biology, regeneration, fibrosis, and disease mechanisms, including cancer and immune dysfunction.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TGFB1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TGFB1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TGFB1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TGFB1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TGFB1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326802285,"sku":"RZ1180","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_28733632-55f0-405d-8a00-a8de5f8a1ac9.jpg?v=1772019451","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-tgfb1-antibody-tgfb1a-tgf-beta-bha17136375","provider":"BioHippo","version":"1.0","type":"link"}