{"product_id":"zebrafish-tnfa-antibody-tnf-alpha-bha17136515","title":"Zebrafish Tnfa Antibody \/ Tnf alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Tnfa Antibody \/ Tnf alpha is a anti-Zebrafish Tnfa Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Zebrafish Tnfa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Tnfa, also known as tumor necrosis factor alpha, is a key pro inflammatory cytokine involved in immune regulation, apoptosis, and host defense. Tnfa is produced mainly by activated macrophages and other immune cells, playing a central role in the inflammatory response and in the activation of downstream signaling pathways such as NF kappa B and MAP kinase cascades. It contributes to both innate and adaptive immunity and is critical for controlling infections, tissue injury responses, and immune cell recruitment.\u003cp\u003eZebrafish Tnfa is an ortholog of the human TNF alpha protein and shares structural and functional conservation with its human counterpart. Similar to human TNF alpha, zebrafish Tnfa is synthesized as a membrane bound precursor that can be cleaved into a soluble form, which binds to TNF receptors to initiate signaling. The conserved nature of Tnfa makes zebrafish a valuable model for studying the role of TNF alpha in inflammatory and immune related diseases.\u003c\/p\u003e\n\u003cp\u003eThere are no confirmed isoforms of zebrafish Tnfa, and its function appears to be largely analogous to the human protein. Research using zebrafish Tnfa provides insights into cytokine signaling, inflammation, immune cell development, and host pathogen interactions.\u003c\/p\u003e\n\u003cp\u003eAntibodies against zebrafish Tnfa protein are useful for applications such as western blot, immunohistochemistry, immunofluorescence, ELISA, and flow cytometry. These tools help researchers study the dynamics of inflammatory signaling and the effects of therapeutic interventions targeting the TNF alpha pathway.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Zebrafish+Tnfa - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Zebrafish+Tnfa - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Zebrafish+Tnfa - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Zebrafish+Tnfa - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zebrafish+Tnfa+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047332012397,"sku":"RZ1321","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ee659336-39b7-465b-8e19-870dab874906.jpg?v=1772019475","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-tnfa-antibody-tnf-alpha-bha17136515","provider":"BioHippo","version":"1.0","type":"link"}