{"product_id":"zebrafish-twist-antibody-twist1a-twist1b-twist2-bha17136516","title":"Zebrafish Twist Antibody \/ Twist1a \/ Twist1b \/ Twist2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZebrafish Twist Antibody \/ Twist1a \/ Twist1b \/ Twist2 is a anti-TWIST1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TWIST1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Twist, also known as twist-related protein, is a key transcription factor that regulates mesodermal and neural development during early embryogenesis. Twist is a basic helix-loop-helix (bHLH) protein that plays a critical role in the process of mesoderm induction, neural tube formation, and muscle development. It is a major regulator of epithelial-mesenchymal transition (EMT), a process essential for cell migration and tissue morphogenesis during development.\u003cp\u003eZebrafish Twist is an ortholog of the human TWIST1 protein, with high sequence conservation and functional similarity. Like its human counterpart, Zebrafish Twist activates target genes that are involved in cell differentiation, migration, and the regulation of cellular plasticity. Mutations or dysregulation of Twist in humans can lead to congenital syndromes, such as Saethre-Chotzen syndrome, which is characterized by craniofacial abnormalities and other developmental defects.\u003c\/p\u003e\n\u003cp\u003eZebrafish Twist exists in multiple isoforms, including Isoform 1a, Isoform 1b, and Isoform 2, each of which may have distinct roles in development. Isoform 1a is typically involved in early developmental processes, such as mesodermal patterning and limb formation, while Isoform 1b and Isoform 2 have been shown to participate in the regulation of more specific aspects of neural crest migration and other specialized cell differentiation pathways. These isoforms provide valuable models for studying the diversity of Twist functions and their roles in development and disease.\u003c\/p\u003e\n\u003cp\u003eThe use of a Zebrafish Twist antibody is valuable for investigating the expression and activity of Twist isoforms in various developmental contexts. These antibodies are useful for applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA to study the spatial and temporal expression patterns of Twist during zebrafish development. A Zebrafish Twist antibody can help researchers understand the role of Twist in regulating cellular behaviors like migration, differentiation, and morphogenesis.\u003c\/p\u003e\n\u003cp\u003eBy using a high-quality Zebrafish Twist antibody, scientists can gain insights into the molecular mechanisms that govern development, as well as the effects of mutations or environmental factors on Twist-related signaling pathways.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TWIST1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TWIST1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TWIST1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TWIST1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TWIST1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331553645,"sku":"RZ1322","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8a3982d4-85b1-413a-8a7d-6b44b54aca67.jpg?v=1772019477","url":"https:\/\/www.ebiohippo.com\/products\/zebrafish-twist-antibody-twist1a-twist1b-twist2-bha17136516","provider":"BioHippo","version":"1.0","type":"link"}