{"product_id":"zyx-antibody-zyxin-bha17135311","title":"ZYX Antibody \/ Zyxin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eZYX Antibody \/ Zyxin is a anti-ZYX Rabbit antibody Recombinant Rabbit Monoclonal clone AFOG-26 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ZYX\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone AFOG-26, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe Zyxin antibody targets Zyxin, a LIM domain-containing cytoskeletal protein encoded by the ZYX gene. Zyxin functions as a scaffold that connects the actin cytoskeleton to signaling pathways controlling cell adhesion, migration, and mechanical stress responses. It localizes to focal adhesions and stress fibers, where it interacts with actin-binding and signaling proteins to regulate cytoskeletal dynamics. The Zyxin antibody enables specific detection of this mechanosensitive protein in studies of cell motility, tissue architecture, and mechanotransduction.\u003cbr\u003e\u003cbr\u003eZyxin is characterized by three LIM domains at its C-terminus, which mediate protein-protein interactions, and a proline-rich N-terminal region that binds to VASP and alpha-actinin. Through these domains, Zyxin coordinates actin filament assembly and cell-matrix adhesion. The Zyxin antibody supports visualization of these interactions, allowing researchers to examine how this structural adaptor transduces mechanical cues into gene expression changes. Zyxin relocates to the nucleus in response to stress, suggesting a dual cytoplasmic and nuclear role in mechanosignaling.\u003cbr\u003e\u003cbr\u003eAs a component of focal adhesions, Zyxin participates in processes such as wound healing, embryonic development, and tissue remodeling. It interacts with proteins including LPP, LIMD1, and Ena\/VASP to modulate cell shape and movement. The Zyxin antibody supports functional studies of these interactions, revealing how Zyxin integrates extracellular matrix forces with actin cytoskeletal organization. Its recruitment to sites of mechanical strain makes it a critical sensor for cellular adaptation to physical stress.\u003cbr\u003e\u003cbr\u003eAltered Zyxin expression has been associated with several diseases, including cancer, cardiovascular dysfunction, and fibrotic disorders. Overexpression can enhance migration and invasion in certain tumors, while loss impairs cytoskeletal resilience. The Zyxin antibody is widely used to investigate its role in metastasis, cell adhesion signaling, and smooth muscle plasticity. Studies have also linked Zyxin to stretch-activated signaling in cardiac and vascular cells, highlighting its contribution to mechanobiology.\u003cbr\u003e\u003cbr\u003eThe Zyxin antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing distinct cytoplasmic and focal adhesion staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ZYX - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ZYX - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ZYX - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ZYX - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ZYX+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047287644525,"sku":"FY12408","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a66f9ed9-baaa-42f5-939d-694c0f2898af.jpg?v=1782237004","url":"https:\/\/www.ebiohippo.com\/products\/zyx-antibody-zyxin-bha17135311","provider":"BioHippo","version":"1.0","type":"link"}