TheE.coli as the host cell is typically used for the prokaryotic expression system for several reasons. The cells have advantages such as rapid proliferation, high expression, easy purification, good stability, strong anti-pollution ability and cost effectiveness. This is why the bacterial expression system is not only the most mature and reliable, but also the most commonly used, economical and effective option for protein expression.
However, there are some difficulties with the prokaryotic expression system. For example, current codon usage bias in CDS or carrier regulatory elements are incompatible with the host bacteria. This may lead to the failure of interest protein expression or low expression. Another issue that may arise is that even if the expression of interest protein succeeds, it may not be soluble.
With years of experience inprotein expression and purification, Synbio Technologies has developed a cost-effective bacterial expression platform to deliver high purity proteins to our customers. By using theNGTM Codon Optimization Technology, the expression level in bacterial can significantly improve. We also offer theSyno® Guaranteed package, which guarantees 3-5 mg recombinant protein of >85% purity to be delivered to our customers.
For those proteins with further special requirements, Synbio Technologies provides you a variety of post processing options. These options include protein tag removal after purification, filtration sterilization, endotoxin removal, lyophilization, N-terminal sequencing and other services.