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Visualizing Cell Death: How E-BLOT Empowered Breakthrough HCC Research
Published On 05/30/2025 7:43 AM
Supplementary Fig. S5 MLKL deficiency disrupts Mg2+ homeostasis in the ER. (Reproduced with permission from Jiang et al., Cell Research, 2023.)Supplementary Fig. S3 MLKL deficiency enhances metabolic stress-induced parthanatos. (Reproduced with permission from Jiang et al., Cell Research, 2023.)
Journal: Cell Research (Nature Publishing Group)
Access: Open Access — Read Here
In A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma (Jiang et al., 2023), researchers discovered that MLKL, best known as a necroptosis executioner, also prevents metabolic-stress-induced parthanatos in liver tumors—helping cancer cells evade immune destruction.
To prove this, they visualized:
Western blotting was central to every molecular conclusion—and the Pro E-BLOT Touch Imager powered it.
“Blots were visualized by Pro e-BLOT Touch Imager according to the manufacturer’s instructions.”
Acknowledgments
Portions of this blog post, including figures and excerpts, are reproduced with permission from Jiang et al., "A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma," Cell Research, 2023.
Read the full study: Cell Research Article
In a recent study published in Cell Research, researchers uncovered a novel role for MLKL in immune evasion within hepatocellular carcinoma (HCC)—and they did it with the help of the Pro E-BLOT Touch Imager.
Advancing Cancer Research: How E-BLOT Enabled Precision Imaging in a Landmark Hepatocellular Carcinoma Study
In the era of immune-oncology breakthroughs, precision at the molecular level isn’t optional—it’s essential. In a major open-access study published in Cell Discovery (Nature Publishing Group), scientists uncovered a new role for MLKL in immune evasion through parthanatos suppression. Their imaging backbone? The Pro E-BLOT Touch Imager.
The Study: A New Chapter in Immune Evasion
Title: A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinomaJournal: Cell Research (Nature Publishing Group)
Access: Open Access — Read Here
In A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma (Jiang et al., 2023), researchers discovered that MLKL, best known as a necroptosis executioner, also prevents metabolic-stress-induced parthanatos in liver tumors—helping cancer cells evade immune destruction.
To prove this, they visualized:
PAR polymer accumulation
Cleaved PARP1
AIF nuclear translocation
MLKL expression across knockouts, knockdowns, and reconstituted lines
Cleaved PARP1
AIF nuclear translocation
MLKL expression across knockouts, knockdowns, and reconstituted lines
Western blotting was central to every molecular conclusion—and the Pro E-BLOT Touch Imager powered it.
Where E-BLOT Shined: Key Figures and What They Show
| Figure 3f: MLKL deficiency enhances metabolic stress-induced parthanatos. (Reproduced with permission from Jiang et al., Cell Research, 2023.) | Target: Poly(ADP-ribose) (PAR), a hallmark of parthanatos Context: Shows enhanced PAR accumulation in MLKL-KO cells upon palmitic acid (PA) treatment E-BLOT Output: Sharp bands, minimal background, quantifiable densitometry-ready signal |
| Supplementary Fig. S3 MLKL deficiency enhances metabolic stress-induced parthanatos. (Reproduced with permission from Jiang et al., Cell Research, 2023.) | Target: MLKL, PAR, GAPDH Insight: Time and dose-dependent accumulation of PAR polymer; supports activation of parthanatos E-BLOT Value: Consistent exposure across blots; side-by-side comparability across conditions |
| Target: MLKL, Calnexin, VDAC, H2A Use Case: Confirms subcellular localization of MLKL in ER fraction E-BLOT Strength: Highly specific banding across cellular compartments; supports ER-Mg²⁺ regulatory model |
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Why This Matters
| Feature | E-BLOT Contribution |
|---|---|
| High sensitivity | Detects post-translational modifications like cleaved PARP1, even at low levels |
| Low background | Enables clean separation between target and non-specific bands |
| Imaging consistency | Supports reproducibility across multiple experiments and figures |
| Publication quality | Accepted in a high-impact Nature family journal without imaging post-processing |
“Blots were visualized by Pro e-BLOT Touch Imager according to the manufacturer’s instructions.”
— Jiang et al., Cell Discovery, 2023
Scientific Impact: Enabling a Discovery
Without the high-quality, quantifiable imaging E-BLOT provided, this study’s key conclusions around MLKL’s RIPK3-independent function in suppressing parthanatos would have lacked visual molecular confirmation. The clarity of E-BLOT’s Western blot images helped:
Validate stress pathway activation
Confirm protein knockouts and reconstitutions
Trace protein localization across cell fractions
In a world where data credibility is everything, E-BLOT delivered.
Ready to Elevate Your Protein Workflows?
Whether you’re investigating immune checkpoints, stress responses, or signal transduction, the Pro E-BLOT Touch Imager delivers the band sharpness, consistency, and publication-grade quality trusted by top-tier researchers.[Request a Demo or Data Sheet]
Acknowledgments
Portions of this blog post, including figures and excerpts, are reproduced with permission from Jiang et al., "A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma," Cell Research, 2023.
Read the full study: Cell Research Article
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