The Baculovirus-Insect Cell Expression System is one of the most popular eukaryotic expression systems for research and industry applications. There are several advantages of using the baculovirus-insect cell expression system, such as improved solubility, the ability to incorporate large DNA fragments, and comprehensive post-translational modifications.
With our experienced scientific team, Synbio Technologies has developed our own baculovirus-insect cell expression technology platform. The production scale of this platform is up to 500 L. Combined with our advanced protein purification facilities, we can deliver recombinant protein products with purity 95% and above. Protein expression levels and success rates can be significantly improved using our proprietaryNGTMCodon Optimization software.
Baculovirus-Insect Cell Expression System Advantages
ProprietaryNGTMCodon Optimization Technology: significantly improve protein expression levels and success rate. Multiple purification strategies: affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc., providing diversified choice of protein purities.
Comprehensive protein identification services: SDS-PAGE, Western Blot, MALDI-TOF, HPLC, etc.
Service Specifications
Steps
Specifications
Deliverables
Timeline
Gene synthesis (optional)
NGTMCodon Optimization Gene synthesis
Plasmid DNA
1-2 weeks*
Subcloning
Subcloning the target gene into expression vector(s)
Constructs
1 week
Baculovirus preparation
Bacmid DNA preparation and infecting insect cell
5ml, >107pfu/ml baculovirus
3-4 weeks
Pilot protein expression and purification
Pilot protein expression and purification Test the expression level
Expression report
1-2 weeks
Large scale protein expression and purification
Scale-up expression and purification
Recombinant protein
QC report
1-2 weeks
* The delivery time of this step bay vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.
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