In classic tissue engineering and regenerative medicine research combines cells and scaffolds, induced to growth with biochemical and mechanical stimuli, in a laboratory setting for organ repair and replacement, AlphaBioGel (PG-‐003) composed extracellular matrix (ECM) fibrous protein molecules secreted by cells. AlphaBioGel (PG-‐003) provides structural and biochemical support to the surrounding cells in 3D tissue models. AlphaBioGel (PG-‐003) increase cell adhesion, cell-‐to-‐cell communication and differentiation. AlphaBioGel (PG-‐003) fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the 3D tissue model, while helping cells build strong basement membranes. In terms of wound healing, AlphaBiogel (PG-‐003) has two main purposes. First, it prevents the immune system from triggering from the injury and responding with inflammation and scar tissue. Next, it facilitates the surrounding cells to repair the tissue instead of forming scar tissue. AlphaBioGel (PG-‐003) can also be used to support 3D cell culture in vitro for modeling tumor development.
Methods
Thaw your AlphaBioGel (PG-‐003) sample bottle at room temp or in a 37ºC water bath, invert product while thawing. When you see a small amount of ice left in your sample, transfer the bottle into an ice bath. It is important to keep AlphaBioGel (PG-‐003) on ice since it will solidify at temperatures above 8ºC.
Precautions
Everything should be kept cold to avoid the AlphaBioGel (PG-‐003) from solidifying. Avoid air bubbles. This protocol is based on 6-‐ well plate usage.