A 1:1000 dilution of SMC-509 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.
Target
Hexanoyl-Lysine adduct
Clone No
500000000
Research Area
Cancer, Oxidative Stress, Lipid peroxidation
Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml
Dilutions
WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.
Purification
Protein G Purified
Scientific Background
Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
Shipping Temperature
Blue Ice or 4ºC
Specificity
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Storage
-20ºC
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Storage Temperature
-20ºC
Trademark
MOLECULAR SIGNATURE®
Reference
1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.
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