Pathway Synthesis

Metabolic Pathway Synthesis and Library Assembly Services

Since the birth of synthetic biology in 2000, metabolic engineering and synthetic biology research have made increasingly huge progress on high value-added natural products. The synthesis and construction of metabolic pathways as well as diverse DNA assembly technologies play fundamental roles in microbiology, biochemistry, and many other relevant fields. With the rapid development of gene synthesis and assembly technology, especially chip-based DNA synthesis technology, the price of large DNA fragment or plasmid synthesis is decreasing year by year, providing opportunities and access for synthetic biologists and metabolic engineers to more easily conduct their research. Over the last 10 years, the continuous development of high flux and automated high-throughput screening methods significantly help to improve the convenience and simplicity of the late screening of library technology, provide more beneficial tools for metabolic engineering and synthetic biology research, and play an important part in chemical synthesis, pharmacology, agricultural, and environmental fields.

• In 2003, Jay Keasling’s team reconstructed the biological synthesis pathway of arteannuinic acid in E. coli, providing functional confirmation for this metabolic process. Over the next decade, they would actively conduct host selection and optimization, taking efforts to achieve product industrialization.
• In 2010, Gregory Stephanopoulos’s team reconstructed the synthesis pathway of Paclitaxel, its precursor, and 5α-hydroxyl paclitaxel, and verified the key four steps during this process, successfully optimizing the metabolic engineering pathway of the Paclitaxel precursor.
• In 2015, Christina Smolke’s team successfully synthesized the precursors of clinically opioid analgesic drugs such as thebaine and hydrocodone in yeast.

Based on Syno^®2.0 and Syno^®3.0 gene synthesis tools and strong long DNA assembly capability, Synbio Technologies is confident in providing professional technical support for metabolic pathway synthesis and library assembly. Specific services include metabolic pathway synthesis and customized metabolic pathway library assembly.

Featured Services

• Our Syno^®2.0 platform is able to provide a fast synthesis of gene fragments and modular genes;
• Our Syno^®3.0 high throughput synthesis platform can assure large-scale reserve of promoters, nucleic acid binding sites, terminators and so on.
• Reliable synthesis of long DNA; a single metabolic pathway has been synthesized to 20Kb
• Wide range of library assembly technologies, allowing for customized library storage

Synbio Technologies case

In this case, the diversity of the promoter library determines the final diversity of the constructed library. The theoretical library capacity is 100, with 100 times coverage, leading to the production of 10,000 clones. Furthermore, the PCR test of the bacterial solution showed 78% positive and 100% accuracy.

Services Specifications

If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.

References：

1. Vincent J J Martin, Douglas J Pitera, Sydnor T Withers, Jack D Newman& Jay D Keasling.Engineering a mevalonate pathway inEscherichia coli for production of terpenoids.Nature Biotechnology 21, 796 – 802 (2003) .
2. Parayil Kumaran Ajikumar, Wen-Hai Xiao, Keith E. J. Tyo, Yong Wang, Fritz Simeon, Effendi Leonard, Oliver Mucha, Too Heng Phon, Blaine Pfeifer, Gregory Stephanopoulos.
3. Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli. Science, 1 October, 2010.
4. Stephanie Galanie, Kate Thodey, Isis J. Trenchard, Maria Filsinger Interrante, Christina D. Smolke. Complete biosynthesis of opioids in yeast. Science, 13 Aug 2015.