CRISPR-Cas9 sgRNA Library

CRISPR-Cas9 sgRNA Library

CRISPR-Cas9/gRNA (CRISPR-Cas RNA-Guided nuclease) is considered as the biggest breakthrough ingenome editing technology after TALEN and ZFN. It precisely edits DNA through the RNA-directed Cas9 nuclease. Previous studies indicated that the on-target gene knockout rates were higher than that with TALEN and ZFN. Besides, the construction of sgRNA-Cas9 is easier and more convenient. It has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. Synbio Technologies offers the designing, construction and validation of CRISPR-Cas9 gene target system through our patent pending platform in a highly cost and time-effective approach. We offer one stop solution for CRISPR Cas9 projects, including genome scale gene targeting.

Advantages of CRISPR-Cas9

• Appliocable in any type of cell lines
• Simultaneously knockout multiple targets
• More efficient
• Fast turnaround time

Applications

• CRISPR-Cas9-based gene knock-out
• CRISPR-Cas9-based gene knock-in
• CRISPR-Cas9-based transcription activation
• CRISPR-Cas9-based transcription inhibition

Services Specifications

• CRISPR-Cas9-based primer design
• CRISPR-Cas9-based plasmid construction
• CRISPR-Cas9-based target locus validation
• CRISPR-Cas9-based stable cell line construction
• CRISPR-Cas9-based stem cell construction

Delivery Form

• Certificate of analysis (COA) for project
• Lyophilized plasmid DNA (2~5 µg), or libraries
• Sequencing chromatogram
• QC report