Lentiviral vectors have a high transfection efficiency on mammalian cells and can provide an efficient way to integrate exogenous gene into host chromosome. As Cas9 protein has a longer ORF, conventional methods (electrotransfection or chemical reagents transfection) have a lower efficiency of inserting Cas9 gene into host cells. Hence, high-efficiency expression lentiviral vectors can be used to construct Cas9-expressing stable cell lines.
Our lentiviral packaging service provides lentiviral expression Cas9/Cas9 Nicknase and Cas9-expressing stable cell lines. It can also enhance the targeting efficiency of Cas9 and enable stable gene expression in host cells. We can make sure the CRISPR-Cas9 system has a higher efficiency of gene editing.
Higher transfection efficiency: Lentiviral vector has a transfection efficiency of nearly 100% on hard-to-transfect cell lines (such as neurons, primary cells, stem cells and tumor cells, etc.).
Large cloning capacity: The longest gene fragment that can be inserted is 8k.
Multigene editing: We can construct Cas9-expressing stable cell lines and enable simultaneously knock-out multiple targets in the same cell.
Cas9 lentiviral vector sequence
Information requested from clients
CRISPR-Cas9 Lentiviral Packaging Service
Strain that needs to be modified along with strain information.
Information about the target cell line along with cell culture condition.
Validated cell line. (Monoclonal cell line is optional)