Ponceau S Protein Staining Solution

Lane 1, 13: Prestained Protein Ladder #PM019-0500

Lane 2-7: 2X Dilutions of Unstained Protein Ladder #PMU12-0500

Lane 8-12: 2000, 1000, 500, 100, 50 ng of BSA

Reaction Setup
 

1. After the SDS-PAGE Gel has been transferred to a nitrocellulose or PVDF membrane, place the transferred membrane in a staining container.
2. Submerge the membrane in a proper amount of Ponceau S Protein Staining Solution that is enough to cover the whole membrane. Lightly agitate the staining container for 5 to 15 minutes at room temperature. Protein bands will be stained and start becoming visible in 5 to 15 minutes.
3. Wash away the excessive stain on the membrane with DI water until the protein bands are clear.
4. For the further membrane blocking step, remove the Ponceau S Staining Solution from the membrane by placing the membrane in 20 ml 0.1% NaOH for 1 to 2 minutes then rinse in DI water for 1 to 2 minutes.