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SKUBHS13100035
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ContentsSynbio Technologies provides comprehensive services for structural biology, including high purity protein preparation, fragment-based...
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Contents
Synbio Technologies provides comprehensive services for structural biology, including high purity protein preparation, fragment-based crystallographic screening, crystallization and structure determination. Synbio Technologies can handle your projects starting from gene synthesis to crystallization/co-crystallization and structure determination. To accomplish this, we rely on our proprietary NGTM Codon Optimization Technology, comprehensive portfolio of expression systems, and a wide selection of purification and crystallization methods.
Phase I:High purity protein production
Milestones Specifications Deliverables Expression Verification and Optimization - Gene synthesis with codon optimization
- Subclone the gene into expression vector(s)
- Expression evaluation
- Expression optimization
Gene in pUC57
Constructs Report
Expression ReportScale-UpProtein Production - Protein expression
- Multi-step purification
- QC by SDS-PAGE, Western blot
High Purity
ProteinPhase II: Crystallization and structure determination
NMR
Milestones Specifications Deliverables NMR Data Collection - 500 MHz Varian
- 600 MHz (cryoprobe) Bruker
- 600 MHz (cryoprobe) Varian
- 800 MHz Bruker
- Probes include 19F and 1H, 2H, 13C, 15N-TR NMR
NMR Data,
including raw data
and processed dataNMR Spectral Analysis - Backbone assignments
- Side-chain assignment
- Protein-Protein, Protein-Nucleic Acid complex analysis
- Dynamics, disorder, stability
- Biosimilarity analysis
MR data, resonance
assignment, reportNMR Solution Structure Determination - Three-dimensional protein structure determination and quality assessment
- Ligand-protein complexes
- Protein-protein complexes
MR data, structure
(PDB), report
including structural
quality assessment statisticsX-ray Crystallography Structures
Milestones Specifications Deliverables Crystallization Screening - Crystallization screening
- Crystallization conditions optimization
Protein crystal X-Ray Data Collection - Collection of high-resolution X-ray data using synchrotron beamline
X-ray data Crystal Structure Determination - Structure determination with crystallography software
Density map,
protein structure
(PDB) and statistics
910 Clopper Rd, Suite 260S
Gaithersburg, 20878
Tel: 1-866-986-9598
United States
[email protected]
Got questions? Call us at 1-866-986-9598
