BJ Fibroblast cell

SKU:BHC11101406
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Overview
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BJ Fibroblast cell is a Fibroblast of foreskin cell line derived from Caucasian (Male). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Fibroblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Morphology Fibroblast
Growth Properties Adherent
Tissue Foreskin
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

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Catalog no. Size
305222 1 cryovial
Field Specification
Species Human
BJ cells, derived from neonatal male foreskin, are human fibroblasts, which are a type of cell found in connective tissue. They are often used in biological and medical research due to their ability to proliferate and their human origin, making them relevant for studying human biology and disease. BJ cells, derived from human skin fibroblasts, are primarily used in studies related to cellular responses to oxidative stress, contributing to our understanding of aging, disease mechanisms, and cellular defense against oxidative damage. The cells further present a viable alternative to mouse BALB/c 3T3 cells for in vitro toxicological evaluations, particularly in the Neutral Red Uptake (NRU) assay. This assay is widely used to assess cytotoxic effects by measuring cell viability through the uptake of neutral red dye. The absence of strong telomerase activity in the BJ human foreskin fibroblasts, independent of hTERT, highlights their role in studying premature senescence, elongation of telomeres, and the effects of hyperoxia on telomere length. The human cell lines BJ and HaCaT are often used together in dermatological research due to their complementary nature in representing key aspects of skin physiology. HaCaT cells, being human keratinocytes, serve as a model for the epidermal layer of the skin, while BJ cells, derived from human fibroblasts, represent the dermal layer. This combination allows for a comprehensive study of skin responses at both the epidermal and dermal levels, making them invaluable for investigating skin aging, wound healing, and the effects of various treatments on skin health. In summary, BJ cells, also known as human BJ fibroblasts, serve as a versatile model in biological research, offering insights into the impact of environmental exposures, cellular senescence, and radical biology.

SKU:BHC11101406

Karyotype: BJ cells maintain a normal diploid karyotype. However, beyond a certain population doubling, an abnormal karyotype indicative of genetic alterations may emerge.

  • cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
  • supplements: Supplement the medium with 10% FBS, 20 ng/mL bFGF
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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