Daudi cell

SKU:BHC11100934
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Overview
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Daudi cell is a B lymphoblast cell line derived from African (Male). It is commonly used as an in vitro model for daudi cells do not release epstein-barr virus (ebv) when cultured, classifying them as risk group 1. however, when used for genetic experiments, they should be treated as risk group 2 cells. research. Growth characteristics: Suspension, Round cells. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Burkitt lymphoma
Morphology Round cells
Growth Properties Suspension
Tissue Blood
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

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Catalog no. Size
302009 1 cryovial
Field Specification
Species Human
The Daudi cell line was established in 1967 from a 16-year-old African boy diagnosed with Burkitt's Lymphoma, a type of lymphoma. Named after the patient from whom it was derived, the Daudi cell line is characterized by its Epstein-Barr Virus (EBV) positivity, a common feature in Burkitt's lymphoma and several other lymphoproliferative disorders. The EBV infection within these cells offers a unique model for studying the virus's role in tumorigenesis, particularly in the context of B-cell malignancies. Daudi human cells lack expression of the classical Major Histocompatibility Complex (MHC) class I molecules on their surface, which is attributed to the absence of beta-2-microglobulin, a crucial component responsible for the correct intracellular folding and processing of the MHC class I molecule in the endoplasmic reticulum. The lack of beta-2-microglobulin in the Daudi cell line leads to a lack of glycosyl modifications necessary for proper cell surface expression of these molecules. The Daudi cell line is extensively utilized in immunology research, particularly in studies involving the immunodepletion of lymphocyte subpopulations, including lymphocytes, natural killer cells, and peripheral blood mononuclear cells. In summary, the Daudi cell line serves as a critical resource for advancing our knowledge in various research fields, from the basic understanding of cell biology to the development of targeted therapies for cancer treatment.

SKU:BHC11100934

  • Antigen expression: CD10+, CD19+, CD20+, CD21+, CD22+, CD23-, CD24-, CD32+, CD37+, CD38+, CD39-,CD40+, CD54+, CD72+, CD73-, CD75+, CD77+, CD81+, CD82+, CD83-, CD84+, CD86+
  • Karyotype: 46, almost diploid
  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% heat-inactivated FBS
  • subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth.
  • seedingDensity: 3 x 105 cells/ml
  • fluidRenewal: 2 times per week
  • postThawRecovery: Fast (48 hours)
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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