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The U87MG cell line, established from a human glioblastoma, is one of the most widely utilized cellular models in neurobiological and cancer research. Originating from a malignant tumor of the central nervous system, these cells exhibit many of the hallmark features of glioblastoma multiforme (GBM), including rapid proliferation, high invasiveness, and significant genetic and phenotypic heterogeneity. This makes the U87MG cell line, also referred to as U87 cells, an invaluable tool for exploring the molecular and cellular mechanisms underlying brain tumors, as well as for testing potential therapeutic strategies.
In neuroscience and immuno-oncology research, U87MG cells serve as a model to elucidate the cell function and cytotoxicity mechanisms in glioblastoma, including the exploration of NK cell cytotoxicity. The expression of NKG2D ligands on U87 cells and the use of NKG2D antibodies in studies highlight the intricate dynamics between cancer cells and the immune system, particularly NK cells, in the tumor microenvironment.
The stemness features of U87 glioblastoma cells, alongside their genetic and phenotypic attributes, are subjects of intense study, aiming to unravel the mechanisms that confer these cells a high degree of plasticity and resistance to conventional therapies. The U87 cell line's exact origin remains somewhat enigmatic, with genetic analyses revealing differences from the original tumor.
In summary, the U87 cell line remains a fundamental tool in glioblastoma research, facilitating a deeper understanding of the disease's biology and the quest for more effective treatments.
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- Isoenzymes: Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B
- Tumorigenic: Yes, in nude mice inoculated subcutaneously with 107 cells
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 4 x 104 cells/cm2
- freezeMedium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
- Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
- Proliferation and Cluster Analysis of Neurons and Glial Cell Organization on Nanocolumnar TiN Substrates
- Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe
- Deep tissue optoacoustic monitoring of photothermal treatments in the NIR-II assisted with silica-coated gold nanorods
- Class I HDAC overexpression promotes temozolomide resistance in glioma cells by regulating RAD18 expression
- Mutant IDH1 Differently Affects Redox State and Metabolism in Glial Cells of Normal and Tumor Origin
- Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications
- Fever-Range Hyperthermia vs. Hypothermia Effect on Cancer Cell Viability, Proliferation and HSP90 Expression
- Cannabinoid receptor CB1 regulates STAT3 activity and its expression dictates the responsiveness to SR141716 treatment in human glioma patients' cells
- ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status
- Deregulated expression and activity of Farnesyl Diphosphate Synthase (FDPS) in Glioblastoma
- Human Microglia Extracellular Vesicles Derived from Different Microglia Cell Lines: Similarities and Differences
- Gold Nanoparticles Enhance Radiosensitivity in Glioblastoma Cells
- The Longevity-Associated Variant of BPIFB4 Reduces Senescence in Glioma Cells and in Patients’ Lymphocytes Favoring Chemotherapy Efficacy
- Spheroid glioblastoma culture conditions as antigen source for dendritic cell-based immunotherapy: spheroid proteins are survival-relevant targets but can impair immunogenic interferon γ production.
- Assessing the potential of bacterial signal peptides for radiopharmaceutical applications.
- Translocator protein (18kDA) (TSPO) marks mesenchymal glioblastoma cell populations characterized by elevated numbers of tumor-associated macrophages.
- New insights into the anticancer activity of carnosol: p53 reactivation in the U87MG human glioblastoma cell line.
- Modified Adenosines Sensitize Glioblastoma Cells to Temozolomide by Affecting DNA Methyltransferases
- Oncogenic relevant defensins: expression pattern and proliferation characteristics of human tumor cell lines.
- PP242 Counteracts Glioblastoma Cell Proliferation, Migration, Invasiveness and Stemness Properties by Inhibiting mTORC2/AKT
- Functional Surfaces Constructed with Hyperbranched Copolymers as Optical Imaging and Electrochemical Cell Sensing Platforms
- PIM kinases mediate resistance of glioblastoma cells to TRAIL by a p62/SQSTM1-dependent mechanism
- Glioblastoma Cells Counteract PARP Inhibition through Pro-Survival Induction of Lipid Droplets Synthesis and Utilization
- Neuronal and glial cell co-culture organization and impedance spectroscopy on nanocolumnar TiN films for lab-on-a-chip devices.
- Human Glioblastoma Multiforme: p53 Reactivation by a Novel MDM2 Inhibitor
- Bax Activation Blocks Self-Renewal and Induces Apoptosis of Human Glioblastoma Stem Cells.
- A Zebrafish Live Imaging Model Reveals Differential Responses of Microglia Toward Glioblastoma Cells In Vivo
- Inhibition of WNT-CTNNB1 signaling upregulates SQSTM1 and sensitizes glioblastoma cells to autophagy blockers
- Polyphenolic Compounds of Crataegus Berry, Leaf, and Flower Extracts Affect Viability and Invasive Potential of Human Glioblastoma Cells
- NMR for screening and a biochemical assay: Identification of new FPPS inhibitors exerting anticancer activity.
- Antitumoral Activity of the Universal Methyl Donor S -Adenosylmethionine in Glioblastoma Cells
- Cyclooxygenase-2 Upregulated by Temozolomide in Glioblastoma Cells Is Shuttled In Extracellular Vesicles Modifying Recipient Cell Phenotype
- Preparation and first biological evaluation of novel Re-188/Tc-99m peptide conjugates with substance-P.
- Large-scale super-resolution optoacoustic imaging facilitated by FeNP/ICG-loaded coreless polyelectrolyte microcapsules
- Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides.
- Bi-specific tenascin-C and fibronectin targeted peptide for solid tumor delivery.
- Human Microglia Extracellular Vesicles Derived from Different Microglia Cell Lines: Similarities and Differences.
- Using Light for Therapy of Glioblastoma Multiforme (GBM)
- A multi-functional fluorescent scaffold as a multi-colour probe: design and application in targeted cell imaging
- Antiglioma effects of N6-isopentenyladenosine, an endogenous isoprenoid end product, through the downregulation of epidermal growth factor receptor.
- N 6 -Isopentenyladenosine Enhances the Radiosensitivity of Glioblastoma Cells by Inhibiting the Homologous Recombination Repair Protein RAD51 Expression
- Lactate dehydrogenase-A inhibition induces human glioblastoma multiforme stem cell differentiation and death
- Origin of the U87MG glioma cell line: Good news and bad news.
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