T2 cell

SKU:BHC11101437
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Overview
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T2 cell is a cell line. It is commonly used as an in vitro model for 2 research. Growth characteristics: Suspension, Lymphoblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Morphology Lymphoblast
Growth Properties Suspension
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

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Catalog no. Size
305228 1 cryovial
Field Specification
Species Human
The T2 cell line is a derivative of the T1 human lymphoblastoid cell line and is characterized by its unique properties related to antigen processing and presentation. These cells are deficient in the transporter associated with antigen processing (TAP), resulting in the inability to effectively transport peptides into the endoplasmic reticulum for loading onto major histocompatibility complex (MHC) class I molecules. This deficiency makes T2 cells particularly valuable in immunological research, especially in studies related to the presentation of antigens and the function of MHC class I molecules. By using T2 cells, researchers can better understand the mechanisms of immune recognition and the role of TAP in antigen presentation. T2 cells are also known for their application in cytotoxic T lymphocyte (CTL) assays. Due to their TAP deficiency, these cells express very low levels of surface MHC class I molecules unless exogenous peptides are added. This property allows for the precise study of peptide-MHC interactions and the evaluation of CTL responses to specific antigens. Furthermore, T2 cells are used in vaccine development research, particularly in designing strategies that enhance the presentation of antigens to the immune system. Their unique characteristics make T2 cells a crucial tool in both basic and applied immunology research.

SKU:BHC11101437

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% heat-inactivated FBS
  • subculturing: Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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