| Field | Specification |
|---|---|
| Species |
HaCaT cells are a pivotal model in dermatological research, offering insights into the complex mechanisms of skin biology and pathology. The spontaneously immortalized HaCaT cell line is derived from adult human epidermal cells and retains the capacity to proliferate and undergo differentiation, similar to basal keratinocytes in vivo. HaCaT cells serve as a robust platform for investigating the epidermal differentiation process and studying the epidermal differentiation markers essential for maintaining skin integrity.
The susceptibility of HaCaT cells to apoptosis and their sensitivity to apoptosis-inducing agents are extensively studied, particularly in the context of cytotoxic agents like RIPL. Researchers assess these agents' cytotoxicities and the extent of cytotoxicity using HaCaT cells, utilizing techniques such as fluorescence microscopy to visualize cellular changes.
Researchers have leveraged HaCaT cells to examine the effects of various agents, including antimicrobial substrates and their influence on cell viability. These cells are an excellent substrate for testing antimicrobial biomaterials and antimicrobial atelocollagen substrates, crucial for skin repair and medical applications.
The HaCaT epidermal line also plays a crucial role in studying cellular senescence, cytokines, and gene expression profiles related to aging and chronic diseases. The transcriptional profiles of HaCaT cells, including the role of κB and microRNAs, provide insight into the regulatory mechanisms at the molecular level.
The HaCaT keratinocyte line, with their characteristics as epidermal keratinocytes, offers a tractable system for dissecting the intricate interplay between epidermal cells and the immune system, specifically the role of keratinocytes in disease states. They enable the exploration of epigenetic modifications and their influence on the differentiation of keratinocytes, including the formation of the cornified envelope, a key feature in the skin's barrier function.
In summary, HaCaT cells are an indispensable model in dermatological research, facilitating a deeper understanding of skin biology and pathology through their resemblance to basal keratinocytes and their ability to undergo cell growth and differentiation. Their application spans from studying epidermal differentiation and antimicrobial effects to exploring cellular responses such as apoptosis, making them a cornerstone in cell biology and biomedical research.
—
- Tumorigenic: No
- Karyotype: Aneuploid (hypotetraploid)
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: The 1:1 mixture of EDTA (stock. 0.05%) and trypsin (stock: 0.1%) must be prepared each time ahead of detaching the cells using PBS without Ca2+ and Mg2+ to provide a physiologic osmolarity. Ready-to-use mixtures of trypsin/EDTA are not recommended, as this may result in cell clumps. As an alternative, TrypLE Express (Life Technologies) instead of trypsin/EDTA can be used. The protocol of the manufacturer should be followed.
- doublingTime: The doubling time of HaCaT cells is 28 hours.
- subculturing:
- Discard Old Medium: Carefully remove the old culture medium from the flasks.
- Wash Cells: Add 3-5 ml of phosphate-buffered saline (PBS) without calcium and magnesium to T25 flasks, or 5-10 ml to T75 flasks, to rinse the adherent cells.
- Add EDTA Solution: Cover the cell layer entirely with a freshly prepared 0.05% EDTA solution. Use 1-2 ml for T25 flasks and 2.5 ml for T75 flasks.
- Incubate: Incubate the flasks at 37°C for 10 minutes.
- Add Trypsin/EDTA or TrypLE Express Solution: After incubation, add a freshly prepared trypsin/EDTA solution (0.05% trypsin, 0.025% EDTA) or TrypLE Express to the flasks, ensuring the cell layer is fully covered. Use 1 ml for T25 flasks and 2.5 ml for T75 flasks. (Note: Steps 3 and 4 can be omitted if using TrypLE Express.)
- Monitor Detachment: Observe the cells under a microscope. The cells should detach within 1-5 minutes.
- Neutralize Trypsin: Add cell culture medium containing fetal bovine serum (FBS) to neutralize the trypsin activity as soon as the cells have detached.
- Transfer Cells: Dispense the cell suspension into new flasks pre-filled with fresh culture medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: 2 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
- Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes
- 2-Hydroxychalcone as a Potent Compound and Photosensitizer Against Dermatophyte Biofilms
- Phytochemical Profiling and Anti-Inflammatory Activity of Rubus parvifolius Leaf Extract in an Atopic Dermatitis Model
- Transient Receptor Potential Ankyrin 1 (TRPA1)—An Inflammation-Induced Factor in Human HaCaT Keratinocytes
- Cutibacterium acnes -macrophage fusion membrane coating Cu/Zn-MOF for psoriasis treatment via pathological innate lymphoid cells inhibition
- Label-free biosensor assay decodes the dynamics of Toll-like receptor signaling
- Structure-based virtual screening of natural compounds in preventing skin senescence: The role of epigallocatechin gallate in protein kinase C alpha-specific inhibition against UV-induced photoaging
- Assessment of cytotoxicity and antioxidant properties of berry leaves as by-products with potential application in cosmetic and pharmaceutical products
- Protective Action of Resveratrol in Human Skin: Possible Involvement of Specific Receptor Binding Sites
- The intracellular seven amino acid motif EEGEVFL is required for matriptase vesicle sorting and translocation to the basolateral plasma membrane
- Oleogel Dressings for Skin Therapy: Physicochemical and Bioactive Properties of Cosmetic Oil-Based Systems Enriched with Essential Oils
- Hepatocyte Growth Factor Activator Inhibitor Type 1 Maintains the Assembly of Keratin into Desmosomes in Keratinocytes by Regulating Protease-Activated Receptor 2–Dependent p38 Signaling
- Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis
- Newly Synthesized CoFe 2−y Pr y O 4 (y = 0; 0.01; 0.03; 0.05; 0.1; 0.15; 0.2) Nanoparticles Reveal Promising Selective Anticancer Activity Against Melanoma (A375), Breast Cancer (MCF-7), and Colon Cancer (HT-29) Cells
- The Splicing Factor PTBP1 Represses TP63 γ Isoform Production in Squamous Cell Carcinoma
- In Vitro Wound Healing Potential of Stem Extract of Alternanthera sessilis
- EPIDERMAL DELETION OF HIF-2α STIMULATES WOUND CLOSURE
- Hydrodynamically generated multilayer skin spheroids enable in vitro screening of biologically active ingredients and toxicity tests
- Camellioside A, isolated from Camellia japonica flowers, attenuates UVA-induced production of MMP-1 in HaCaT keratinocytes via suppression of MAPK activation
- Efficacy of Pre- and Post-Treatment by Topical Formulations Containing Dissolved and Suspended Silybum marianum against UVB-Induced Oxidative Stress in Guinea Pig and on HaCaT Keratinocytes
- Evaluation of the Efficacy of Xyloglucan, Pea Protein and Opuntia ficus-indica Extract in a Preclinical Model of Psoriasis
- Waste Citrus limon Leaves as Source of Essential Oil Rich in Limonene and Citral: Chemical Characterization, Antimicrobial and Antioxidant Properties, and Effects on Cancer Cell Viability
- Exploring Cannabidiol (CBD) and Cannabigerol (CBG) Safety Profile and Skincare Potential.
- Deciphering Staphylococcus aureus- host dynamics using dual activity-based protein profiling of ATP-interacting proteins
- Brown Pine Leaf Extract and Its Active Component Trans -Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K
- A Randomized Double‐Blind Controlled Evaluation of the Therapeutic Benefits of an Herbal Lip Hydrant
- Modulation of Corticotropin-Releasing Hormone Receptor Expression During In Vitro Keratinocyte Differentiation
- Herpes simplex virus downregulation of secretory leukocyte protease inhibitor enhances human papillomavirus type 16 infection
- Titanium-Integrated Magnetic Silica Aerogels via Microfluidic Synthesis for Pesticide Removal from Water
- Identification of senescence rejuvenation mechanism of Magnolia officinalis extract including honokiol as a core ingredient
- A comparative study on the biological activity of essential oil and total hydro-alcoholic extract of Satureja hortensis L.
- 2-Methoxystypandrone from Polygonum cuspidatum Rejuvenates Senescence by Reducing Mitochondrial ROS
- Triggering Apoptotic Death of Human Epidermal Keratinocytes by Malic Acid: Involvement of Endoplasmic Reticulum Stress- and Mitochondria-Dependent Signaling Pathways
- Exploring Anti-Aging Potential of Dendrobium Species and Novel Microemulsion Delivery of Dendrobium kentrophyllum Extract for Anti-Aging Effect
- Differential Expression of Keratinocyte-Derived Extracellular Vesicle Mirnas Discriminate Exosomes From Apoptotic Bodies and Microvesicles
- A Novel Multi-Component Formulation Reduces Inflammation In Vitro and Clinically Lessens the Symptoms of Chronic Eczematous Skin
- Robust polyfunctional CD8+ and CD4+ T cell responses in HLA-A*0201/DR1 transgenic mice following vaccination with modified vaccinia virus Ankara-based vaccines delivering Lassa virus glycoprotein or nucleoprotein
- Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation
- Aspergillus oryzae -Fermented Wheat Peptone Enhances the Potential of Proliferation and Hydration of Human Keratinocytes through Activation of p44/42 MAPK
- Differential Effects of Histidine and Histidinamide versus Cysteine and Cysteinamide on Copper Ion-Induced Oxidative Stress and Cytotoxicity in HaCaT Keratinocytes
- Evaluation of Cedrus atlantica Essential Oil: Chemical Composition, Anticancer Activity and Molecular Docking Studies
- Optimization of Natural Deep Eutectic Solvent-Assisted Extraction of Rosmarinic Acid from Thunbergia laurifolia Lindl. and Evaluation of Antioxidant Activity
- Prediction of drug-induced liver injury using keratinocytes
- Antioxidant and Anti-Inflammatory Activities of Sargassum macrocarpum Extracts
- Anti-biofilm Action of Chenopodium ambrosioides Extract, Cytotoxic Potential and Effects on Acrylic Denture Surface
- Fe 3 O 4 @β-cyclodextrin Nanosystem: A Promising Adjuvant Approach in Cancer Treatment
- Protective Effects of Sesame Glycoproteins on Ultraviolet-Induced Skin Aging: In Vitro and In Vivo Studies
- The S100A10 Subunit of the Annexin A2 Heterotetramer Facilitates L2-Mediated Human Papillomavirus Infection
- Poly(chitosan-ester-ether-urethane) Hydrogels as Highly Controlled Genistein Release Systems
- Phoyunnanin E inhibits migration of non-small cell lung cancer cells via suppression of epithelial-to-mesenchymal transition and integrin αv and integrin β3
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
