EZ Set Accessory Kit

Background

Sandwich ELISA is a widely used immunoassay format for quantifying proteins in complex biological matrices. In a typical workflow, a capture antibody immobilizes the analyte, and a second detection antibody provides specificity for signal generation. Accessory reagents and standardized consumables can reduce day-to-day variability and make it easier to scale DIY ELISA development across multiple targets.

Why accessory components matter

When building custom ELISAs, consistent plate handling, washing, blocking, and detection chemistry are essential for reliable calibration curves and comparable results across experiments. Using a fixed accessory set helps keep non-target variables constant so that optimization focuses on target-specific parameters such as antibody pairing, standard selection, and matrix effects.

Interpreting ELISA data

Quantitative readouts should be interpreted in the context of sample preparation, dilution strategy, and biological variability. Including appropriate controls and replicates supports robust comparisons across conditions and time points.

• Datasheet (PDF)
• MSDS (MSDS)

Kit components

Materials required but not provided

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
13. PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
14. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
15. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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