Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

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Mfr.No.

CSB-EL018125HA

Sensitivity

3.12 pg/mL

Detection Range

12.5 pg/mL-800 pg/mL

Reactivity

Golden hamster

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

phospholipase B domain containing 2, P76, LAMA-like protein 2, PLB homolog 2, lamina ancestor homolog 2, mannose-6-phosphate protein associated protein p76, phospholipase B-like 2 32 kDa form, phospholipase B-like 2 45 kDa form

Recovery

The recovery of mouse C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	100
	Range %	94-106
1:2000	Average %	89
	Range %	83-96
1:4000	Average %	93
	Range %	87-99
1:8000	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
6000	2.203	2.036	2.120	2.034
3000	1.365	1.276	1.321	1.235
1500	0.764	0.704	0.734	0.648
750	0.425	0.397	0.411	0.325
375	0.287	0.294	0.291	0.205
187.5	0.212	0.196	0.204	0.118
93.75	0.156	0.159	0.158	0.072
0	0.087	0.084	0.086	?

Species

Mesocricetus auratus

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PLBD2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500875	CSB-EL018125HA	96T	$790	Add to Cart
BHE10500875	CSB-EL018125HA	5x96T	$3792	Add to Cart
BHE10500875	CSB-EL018125HA	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.