| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily that signal through type I and type II serine/threonine kinase receptors. Ligand binding activates the receptor-regulated SMADs (SMAD1, SMAD5, and SMAD8), which form a complex with the common mediator SMAD4 and accumulate in the nucleus, where they bind BMP response elements (BRE) to regulate target gene transcription. BMP signaling controls embryogenesis, skeletal and organ development, stem cell fate, and adult tissue homeostasis. Dysregulated BMP signaling is associated with skeletal disorders, fibrosis, and cancer, making BRE-driven reporters useful tools for studying this pathway.
Product Description & Applications
The BRE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of BMP signaling pathway activity in mammalian cells. The construct contains inverted repeats of BMP response elements from the Id2 promoter plus a SMAD4 DNA-binding motif; upon BMP pathway activation, the SMAD1/5/8-SMAD4 complex binds the BRE and drives reporter expression. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, luminometry, or β-galactosidase assay. The system is applied to study BMP signaling in development, stem cell differentiation, and tissue homeostasis. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP, β-Galactosidase reporter gene under the control of tandem consensus response elements specific for the BMP Signaling Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
