| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
C/EBP-α (CEBPA) is a basic leucine zipper transcription factor and a key regulator of cell differentiation and metabolism. By binding CCAAT-box sequences in target gene promoters, it controls the differentiation of adipocytes, hepatocytes, and granulocytes, and coordinates genes involved in lipid and glucose metabolism and energy balance. C/EBP-α also restrains cell proliferation, helping couple differentiation to cell-cycle exit. In adipocyte and hepatic biology, C/EBP-α acts together with PPAR pathway factors to drive metabolic gene programs. Loss-of-function mutations in CEBPA are recurrent in acute myeloid leukemia, making it a target of interest in metabolism, differentiation, and cancer research.
Product Description & Applications
The C/EBP-α Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of C/EBP-α transcriptional activity in human or mouse cells. Reporter expression is controlled by C/EBP-responsive elements, so signal intensity reflects endogenous C/EBP-α pathway activation. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study C/EBP-α function in adipocyte and hepatocyte differentiation, metabolic gene regulation, and myeloid biology. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the C/EBP-a, PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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